摘要
本实验观察到HIP_2、APT_4和HI1173种刺激型McAb均能使PRP中血小板细胞骨架蛋白的主要成分肌动蛋白(actin)含量增加,而钙调蛋白抑制剂(EBB)则能显著抑制HIP_2和APT_4引起的肌动蛋白增加,抑制率分别为76.3％和48.95％,但不抑制HI117引起的肌动蛋白增加。此外,EBB能完全抑制APT_4引起的血小板聚集,部分抑制HIP_2引起的血小板聚集,不抑制HI117引起的血小板聚集。将3种刺激型McAb加入经0.3mmol/L EDTA溶液洗涤过3次的血小板悬液中,无透光度改变,也没有肌动蛋白动员,提示此3种McAb引起的血小板聚集及肌动蛋白动员除与Ca^(2+)有关外,还与血浆中某种(些)因子有关,Ca^(2+)—钙调蛋白系统在HIP_2,APT_4引起的血小板聚集及肌动蛋白动员中起着重要的作用。
The effects of monoclonal antibodies HIP_2, APT_4 and HI117 on the platelet cytoskeleton were investigated. The results showed that HIP_2, APT_4 and HI117 could induce an increase of platelet cytoskeloton materials in PRP. The action of HIP_2 and APT_4 could be obviously inhibited by the calmodulin inhibitor EBB. The inhibition rates of EBB were 76.3％ and 48.95％, respectively. But EBB couldn't inhibit the HI117-induced an increase of platelet cytoskeleton materials. EBB could completely inhibit APT_4-induced platelet aggregation(100％) and partially inhibit HIP_2-induced platelet aggregation (14.26％).It couldn't inhibit HI117-induced platelet aggregation. These McAbs couldn't induce aggregation and actin mobilization in washed platelets in the presence of EDTA. The results indicate that platelet aggregation and the increase of actin induced by these McAbs, in addition to Ca^(2+), could be related to a factor or factors in the plasma. The Ca^(2+)-CM system might play an important role in platelet aggregation and actin mobilization induced by HIP_2 and APT_4.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1992年第1期1-5,共5页
Acta Academiae Medicinae Sinicae
基金
国家自然科学基金
关键词
抗体
血小板聚集
细胞骨架蛋白
antibodis,monocolonal
platelet aggregation
cytoskeleton
