摘要
目的 :建立红景天注射剂 (红景天 )的HPLC指纹图谱。方法 :选择YWG C18色谱柱 ( 4 .6mm× 15 0mm 10 μm) ,流动相 (A)甲醇 乙腈 ( 1∶1)、(B) 0 .0 7%磷酸水梯度洗脱 ,运行 6 0min ,检测波长 2 78nm ,测定红景天药材、中间体及注射剂的指纹图谱。结果 :确定了注射剂的 17个共有峰 ,并得到了以红景天苷为内标的相对保留时间及峰面积比值 ,非共有峰面积未超过总峰面积的 10 %。结论 :方法简单可靠 ,可作为红景天注射剂的质量控制指标。
AIM: To establish the fingerprint chromatogram of Hongjingtian Injection ( Rhodiola kirilowii (Regel.) Maxim. METHODS: HPLC with YWG C 18 column was used, the (a)MeOH ACN(1∶1)、(b) 0.07% H 3PO 4 H 2O gradient elution as a mobile phase and detection wavelength at 278nm . RESULTS: 17 peaks were indicated on the HPLC fingerprint of Hongjingtian Injection. The relative retention time and relative peak area were obtained with itself peak. CONCLUSION: The method is simple and accurate with good reproducibility and can be used as a quality control method of Hongjingtian Injection.
出处
《中成药》
CAS
CSCD
北大核心
2004年第4期259-261,共3页
Chinese Traditional Patent Medicine
关键词
红景天注射剂
红景天
指纹图谱
HPLC
Hongjingtian Injection
Rhodiola kirilowii (Regel) Maxim
fingerprint chromatogram
HPLC
