摘要
本文报告了一个可同时测定卡马西平及其少在40mg·L^(-1)内呈线性,1O,11一环氧卡马西平活性代谢产物血药浓度的高效液相色谱法。于至少在20mg·L^(-1)内呈线性。0.3ml血清样本中加入内标物(10—甲氧卡马西平)后.经4mol·L^(-1)氢氧化钠碱化.用二氯甲烷提取;提取液在室温中氮气流下挥干、流动相重组进样.进行色谱分析。以乙腈/甲醇/水(50/210/260.V/V)为流动相.以C_(18)反相桂(150×4.6mm)为固定相,检测波长为214nm。本法卡马西平及1O,11—环氧卡马西平的最小检出浓度分别为0.08mg·L^(-1)和0.1mg·L^(-1).平均绝对回收率分别为96.0％和97.3％;日内变异系数为3.3％～5.7%.日间变异系数为4.3％～9.0％;
An isocratic high-performance liquid-chromatographic method is-described for the simultaneously determining carbamazepine and its biologically active metabolite, carbamazepine-10, 11-epoxide. Serum samples containing an internal standard (10-methoxycarba-mazepine) are extracted with dichlor-methane and 4 mol · L-1 sodium hydroxide. The organic extract are evaporated at ambient temperature under a stream of nitrogen. The residues are reconstituted in mobile phase and injected on to a re-versed-phase C18 Column (150×4. 6mm I. D). The mobile phase consists of ace-tontrile/methanol/water (50/210/260 by vol) . The UV detector is operated at 214nm. The elution times of all com-
pounds are within 7 min.
The least detectable concentrations of carbamazepine and carbamazepine-10, 11-epoxide are 0. 08mg · L-1 and 0.1mg · L-1, respectively. The average absolute recovery for carbamazepine is 96.0% and for carbamazepine-10,11-epoxide is 97.3%. Within-run CV is 3. 3%~5.7%. and between-run CV is 4.3%~9.0%. The standard curve is linear at least within 40mg · L-1 for carbamazepine and within 20mg · L-1 for carbamazepine-10, 11-epoxide.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1992年第5期393-397,共5页
Chinese Pharmacological Bulletin
关键词
高效液相色谱
卡马西平
carbamazepine
carba- mazepine-10, 11-epoxide
high-performance liquid-chromatographic method
therapeutic drug monitoring.
