摘要
采用萃取法分离-离子对萃取-比色法和异羟肟酸反应-三氯化铁显色-比色法分别测定了乌头中毒性生物碱乌头碱类(A)和酯型生物碱的含量。根据以上测得结果求得了苯甲酰乌头原碱类(B)的含量,进而求得去毒指数(DI,DI=B/(A+B))。采用上下法和醋酸诱导扭体法分别测定乌头对小鼠的LD_(50)和半数镇痛效量(AD_(50)),进而求得了治疗指数(TI,TI=LD_(50)/AD_(50))。DI生品为3.83%,炮制品为51.9～70.3%;TI分别为3.2,4.0～7.6。结果表明DI可用来评估乌头的有效性和毒性。
The content of toxic alkaloids aconitines (A) and ester-alkaloids in the main roots of Aconitun carmi Chaeli Debx. was determined by extraction separation-ion pair extraction-colorimetry and hydroxamic acid reaction-ferric chloride coloring-colorimetry respectively. According to the analytical results mentioned above, the contents of benzoylaconines (B) and detoxification index (DI, DI=B/(A+B)) were obtained. By using up-and-down method and acetic acid-induced writhing method, LD_(50) and median analgesic dose (AD_(50) of aconites in mice was assayed, and then the therapeutic index (TI, TI=LD_(50)/AD_(50)) was achieved. DI of raw or processed aconites was 3.83%, or 51.9～70.3%; and TI was 3.2, 4.0～7.6 respectivly. It was found that DI can be applied to estimate both therapeutic effect and toxicity of aconites.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1992年第4期229-233,共5页
Journal of China Pharmaceutical University
关键词
乌头
中药炮制
毒性
乌头碱性
比色法
Aconi■n carmichaeli Debx
Traditional Chinese medicines processing
Toxicity
Aconitine
(?)metry
