摘要
目的 :探讨高效液相色谱法测定当归中阿魏酸含量的方法。方法 :以 1 %冰醋酸溶液为阿魏酸对照品的溶剂 ,40 %甲醇溶液为样品溶剂 ,固定相为BatesilC1 8(2 50mm× 4 6mm ,5μm) ;流动相为甲醇∶1 %冰醋酸∶甲氢呋喃 (30∶70∶1 ) ;检测波长 32 0nm。结果 :阿魏酸在 3 0 5～30 5μg/ml范围内线性良好 (r=0 9999,n =5) ,平均加样回收率为 98 8% ,RDS为 1 0 7% (n =9)。结论 :高效液相色谱法可用于当归药材中阿魏酸含量的测定。本方法简单准确 ,灵敏度高。
Objective:To determine ferulic acid content in angelia sinensis(Oliv.)diels.Methods:1% Acetic acid was used as solvent for ferulic acid and 40%.Methanol was used as solvent for angerlia sinensis.ARP-HPLC method that determined the ferulic acid content in Angelica Sinensis was set up.The HPLC with BATESIL C 18(4.6× 250 mm,5-Micro)column was used,the Methanol:Tetrahydrofuran:1% Acetic acid=30∶1∶70 as a mobile phase and detection wavelength at 320 nm.Results:A good linearity was obtained from 3.05～ 30.5 μg·ml -1with r=0.9999 for ferulic acid.The average recovery was 98.8%,RSD=1.07%(n=9).Conclusions:HPLC and determinate ferulic acid content in angelia sinensis(Oliv.) diels.The method was proved to be simple,precise,sensitive.
出处
《中国冶金工业医学杂志》
2004年第2期96-97,共2页
Chinese Medical Journal of Metallurgical industry
关键词
高效液相色谱法
含量测定
当归
阿魏酸
植物药
Ferulic acid Angerlia sinensis(Oliv.)diels HPLC Content determinate
