摘要
目的 :探讨环孢素A对脑缺血再灌注损伤的保护作用。方法 :将 10 8只大鼠分为假手术组、对照组和环孢素A组 ,参照Zealonga线栓法制备局灶性脑缺血再灌注损伤模型 ,大鼠脑缺血 2h再灌注 2 2和 70h后 ,分别对各组各时间点大鼠进行行为学评分、脑组织含水量、脑TTC染色和脑超微结构观察 ,并对结果进行统计处理。结果 :各时间点环孢素A组与对照组比较 ,行为学评分优于对照组 (P <0 .0 5 ) ;脑梗死灶体积和脑组织含水量均比对照组明显减轻 (P <0 .0 5 ) ;脑超微结构的异常改变轻于对照组 ;假手术组各项观察指标则无明显异常改变。结论 :免疫因素参与脑缺血再灌注损伤 ;环孢素A对大鼠脑缺血再灌注损伤具有一定的保护作用。
Objective: To explore the protective effect of cyclosporin A on cerebral ischemia-reperfusion injury in rat. Methods: 108 Wistar rats were divided into 3 groups, sham operation group, saline control group and cyclosporin A treatment group. The focal cerebral ischemia-reperfusion injury model was made by suture-occluded method. The score of behavior obstacles of rats were evaluated. The cerebral water content was determined by method of dry-wet weight. TTC staining was used to calculate the cerebra infarct volume. The morphological changes of cerebra were observed under an electron microscope. Results: In the scheduled time, the score of behavior obstacles in cyclosporin A group was higher than in saline group ( P <0.05); both the infarct volume and the cerebral water content in cyclosporin A group were significantly lower than in the saline group ( P <0.05); the electron microscopy showed that the cerebral morphological changes were milder in cyclosporin A group than in saline group; the sham operation group had no obvious abnormal change in each observational item. Conclusion: The immune factors may play an important role in cerebral ischemic reperfusion injury of rats. Cyclosporin A could have protective effects on cerebral ischemic reperfusion injury in rats.
出处
《中国康复》
2004年第2期70-72,共3页
Chinese Journal of Rehabilitation
关键词
环孢素A
脑缺血再灌注
大鼠
cyclosporine A
cerebral ischemia-reperfusion
rat
