摘要
目的 探讨病毒性脑炎患儿血清及脑脊液中一氧化氮 (NO)、一氧化氮合成酶 (NOS)的变化及临床意义。方法 采用ELISA方法 ,分别检测 5 7例〔其中 ,男 31例 ,女 2 6例 ,年龄 9月～ 12岁 ,平均 7 2岁〕病毒性脑炎患儿血清及脑脊液中和 2 3例健康儿〔其中 ,男 14例 ,女 9例 ,年龄 4～ 12岁 ,平均 8 2岁〕血清中NO、NOS的含量 ,对比观察急性期、恢复期以及不同病原体脑炎之间血清及脑脊液中NO、NOS含量的变化。结果 患儿急性期血浆中NO、NOS分别为 6 75± 2 2 4ng/ml和 34 5 6± 7 82U/ml明显高于恢复期的 4 4 7± 2 14ng/ml和 2 4 6 5± 6 84U /ml(P <0 0 1)和高于对照组的 (3 78± 0 74 )ng/mland (17 86±4 5 4)U/ml(P <0 0 1) ;急性期脑脊液中NO、NOS分别为 7 5 6± 2 2 4ng/ml和 36 5 6± 7 88U/ml明显高于恢复期的 4 2 6± 2 0 2ng/ml和 2 6 35± 4 78(P <0 0 1)流行性腮腺炎病毒性脑炎病人血浆、脑脊液中NO、NOS的含量与其他不同病原体脑炎中的含量相比无明显差异。结论 病毒性脑炎急性期血浆、脑脊液中NO及其合成酶生成增加 ,但不同病原体脑炎中的含量变化相比无明显差异 ,提示NO作为内源性调质 ,参与病毒性脑炎的病理生理过程。
Objective To investigate The Changes and effects of nitric oxide and its synthase in the serums and cerebrospinal fluids on Patients with viral encephalitis.Methods nitric oxide and its synthase in the serums and cerebrospinal fluids of 57 Patients with viral encephalitis (31 boys and 26 girls), aged 7 months-12years,average(7.2±1.3)years and 23 healthy children (control group,14 boys and 9 girls, aged 5-12 years, average (8.2±1.5)years) were measured with ELISA and were compared with groups divided.Results The concentrations of the acute stage serums NO and NOS of patients with viral encephalitis ( 6.75±2.24)ng/ml and (34.56±7.82)U.ml were significantly higher than the convalescent serum 4.47±2.14 ng/ml and 24.65±6.84U/ml(P<0.01) and the control group(3.78±0.74)ng/ml and (17.86±4.54)U.ml(P<0.01),The concentrations of the acute stage csf NO and NOS of patients with viral encephalitis (7.56±2.24ng/ml and 36.56±7.88 U.ml)were significantly higher than the convalescent cerebrospinal fluids (4.26±2.02 ng/ml and 26.35±4.78(P<0.01)).Conclusion These results indicated that NO?NOS are in a status of being highly level in patients with viral encephalitis and suggested that NO is involved in viral encephalitis processes.
出处
《济宁医学院学报》
2004年第1期23-24,共2页
Journal of Jining Medical University
基金
山东省卫生厅资助课题 (编号 2 0 0 2 -0 17)
