摘要
目的 采用变性梯度胶电泳方法 (DGGE)研究喂养方式对早产新生儿肠道菌群的影响。方法 收集同期 6对新生儿 1～ 2 1d粪便 ,直接提取细菌总DNA ,扩增 16SrDNAV6～V8区后DGGE分离 ,测序并与EM BL核苷序列数据库进行比较。结果 喂养前肠道菌群类似 ,以梭状芽孢杆菌、链球菌、克雷伯氏菌为主 ,开奶后母乳喂养儿以双歧杆菌为主 ,奶粉喂养儿肠道菌群显示其明显的多态性 ,有双歧杆菌、梭状芽孢杆菌、链球菌、大肠杆菌、克雷伯氏菌、韦荣氏菌、沙雷氏菌以及不经培养细菌。结论 喂养方式对早产新生儿菌群的形成及演替有明显影响 ,PCR -DGGE在多态性 ,动力性 ,菌群的演进变化方面提供了更加准确的数据和补充资料。
Objective:To study the difference of intestinal microbiota between breast-feeding and formula-feeding premature neonates. Methods:Total DNAs were extracted from the feces of two group neonates. Bacterial communities in two group infants were examined by PCR of 16S rDNA V6-V8 regions and denaturing gradient gel electrophoresis(DGGE) and by analyzing the sequences of the ribotypes. Results: During the first few days of life the profiles of DGGE were simple, but they became more complex as the bacterial diversity increased with time, especially in formula-feeding infants. Species most closely related to the Bifidobacterium dominated the intestinal microbiota in breast-feeding infants. Bacterial diversity were more marked in formola-feeding infants than breast-feeding.the bacteria identified included members of genera Bifidobacteria, Clostridium, E.coli, Streptococcus, Uncurtured bacteria, Klesiella, Veillonella, Uncultured fecal bacterium, Serratia.Conclusion: Feeding mode is a important factor affecting developmental intestinal microbiota in premature neonates.PCR-DGGE and 16S rDNA sequence analysis resulted in a dynamic description of bacterial ciloniztion in the infant intestinal microbiota and allowed visualization of bacteria that are difficult to cultivate or be detected by other methods.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第7期57-61,共5页
China Journal of Modern Medicine
