摘要
AIM:To investigate the effect of Helicobacter pylori (H pylon)infection on the expressions of Bcl-2 family members in gastric adenocarcinoma.METHODS:Gastric adenocarcinoma and resection margin tissues of 95 patients were studied.Semi-quantitative RT-PCR was used to measure Bid, Bax and Bcl-2 mRNA expressions.RESULTS:Expressions of Bid and Bax in gastric adenocarcinoma tissues without H pyloriinfection,with cagA^- H pyloriinfection and cagA^+ H pyloriinfection increased significantly in turn (Bid,0.304,0.422 and 0.855 respectively, P<0.05, Bax,0.309,0.650 and 0.979 respectively, P<0.05). Bcl-2 mRNA levels increased significantly in gastric adenocarcinoma tissues with cagA^- H pylori infection and cagA^+ H pylori infection,compared with those without H pyloriin fection(0.696 and 0.849 vs0.411, P<0.05).Expressions of Bid,Bax and Bcl-2 in resection margin tissues without Hpylori infection,with cagA^- H pylori infection and cagA^+ H pylori infection increased significantly in turn (Bid, 0.377, 0.686 and 0.939 respectively, P<0.05, Bax,0.353,0.645 and 1.001 respectively,P<0.05,Bcl-2,0.371,0.487 and 0.619 respectively,P<0.05).In H pylori negative specimens,expressions of Bid and Bax correlated negatively with that of Bcl-2 respectively in adenocarcinoma tissues (Bid vs Bcl-2, r=-0.409, P<0.05,Bax vs Bcl-2,r=-0.451, P<0.05).In H pylori positive specimens,expressions of Bid and Bax did not correlate with that of Bcl-2 in adenocarcinoma tissues(Bid vs Bcl-2,r=-0.187, P>0.05,Bax vs Bcl-2,r=0.201,P>0.05),but correlated positively with that of Bcl-2 respectively in resection margin tissues (Bid vs Bcl-2,r=-0.331,P<0.05,Bax vs Bcl-2,r=-0.295, P<0.05).CONCLUSION:H pylorimay enhance Bid, Bax and Bcl-2 mRNA levels and cause deregulation of these apoptosis-associated genes expressions, which may play a role during development of gastric adenocarcinoma induced by H pylori.
AIM:To investigate the effect of Helicobacter pylori (Hpylori) infection on the expressions of Bcl-2 family members in gastric adenocarcinoma. METHODS:Gastric adenocarcinoma and resection margin tissues of 95 patients were studied.Semi-quantitative RT-PCR was used to measure Bid,Bax and Bcl-2 mRNA expressions. RESULTS:Expressions of Bid and Bax in gastric adenocarcinoma tissues without H pylori infection,with cagA^- H pylori infection and cagA^+ H pylori infection increased significantly in turn (Bid,0.304,0.422 and 0.855 respectively,P<0.05; Bax, 0.309,0.650 and 0.979 respectively,P<0.05).Bcl-2 mRNA levels increased significantly in gastric adenocarcinoma tissues with cagA^- Hpylori infection and cagA^+ Hpylori infection,compared with those without Hpylori infection (0.696 and 0.849 vs 0.411,P<0.05).Expressions of Bid, Bax and Bcl-2 in resection margin tissues without Hpylori infection,with cagA^- H pylori infection and cagA^+ H pylori infection increased significantly in turn (Bid,0.377,0.686 and 0.939 respectively,P<0.05; Bax,0.353,0.645 and 1.001 respectively,P<0.05; Bcl-2,0.371,0.487 and 0.619 respectively,P<0.05).In H pylori negative specimens, expressions of Bid and Bax correlated negatively with that of Bcl-2 respectively in adenocarcinoma tissues (Bid vs Bcl- 2,r=-0.409,P<0.05; Bax vs Bcl-2,r=-0.451,P<0.05).In H pylori positive specimens,expressions of Bid and Bax did not correlate with that of Bcl-2 in adenocarcinoma tissues (Bid vsBcl-2,r=-0.187,P>0.05; Bax vs Bcl-2,r=0.201, P>0.05),but correlated positively with that of Bcl-2 respectively in resection margin tissues (Bid vs Bcl-2,r=0.331,P<0.05; Bax vs Bcl-2,r=-0.295,P<0.05). CONCLUSION:H pylori may enhance Bid,Bax and Bcl-2 mRNA levels and cause deregulation of these apoptosis- associated genes expressions,which may play a role during development of gastric adenocarcinoma induced by H pylori.
基金
Supported by the National Natural Science Foundation of China,No.30070043,and the Key Programs of the Military Medical and Health Foundation during the 10th Five-Year Plan Period,No.O1Z075
