摘要
用激光薄层密度扫描仪对171份血清补体 C4的电泳色带进行扫描,并求取其 C4A:C4B 比值(R 值)。结果发现含 C4A·QO 基因者的 R 值范围0.068～0.429,平均0.228,而含 C4B·QO 基因者的 R 值范围2.197～9.334,平均3.973;在所检出的7例具有 C4B·座位重复基因的样本中.其 R 值均很小(0.120～0.625),且其中含有 C4A·QO 者与不含 C4A·QO 者的 R 值有明显差异.结果表明根据 R 值大小来指定C4·QO 基因和重复基因是有一定道理的.在最常见的 C4表型3,3/1,1中,其 R 值呈常态分布,但表型3,3/2,1、3,3/2,2和3,2/1,1的 R 值多大于1.0,而表型4,4/2,2和4,4/2,1的 R 值多小于1.0。两组表型的 R 值之间的两两比较有显著性差异(P<0.01)。
The human complement C4 electropheresis bands of 171 serem samples were scaned with UltroScan aser Densitometer(LK.B2202),and the ratio of C4A to C4B of(R value)of each sample was calculated.It was found that he R values of samples with C4A~* QO range from 0.068 to 0.429 with an average of 0.228,while the R values of amples with C4B~* QO range from 2.197 to 9.334 with an average of 3.973.The R values of 7 samples with duplicated genes at C4B locus were very small(range from 0.120 to 0.625),and among them,the R values of samples with 4A~*QO were significantly different from that of samples without C4A~* QO.This results indicated that it is reasonable to assignate C4~* QO gene and duplicated gene according to the R value of each sample.In addition,we have found that among the most common C4 phenotype 3,3/1,1,the R values were shown as normal distribution,but the R values of C4 phenotypes 3,3/2,1,3,3/2,2 and 3, 2/1,1 were mostly above 1.0,while the R values of 4,4/2,2 and 4,4/2,1 were mostly below 1.0.The differences between R values of C4 phenotypes in these two groups were signifficant(P<0.01).
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1992年第2期72-76,共5页
Chinese Journal of Immunology
基金
国家自然科学基金
卫生部政策性资助课题
中澳教育合作项目
关键词
补体C4
表型
遗传多态性
补体型
Complement C4
Phenotype
Genetic polymorphism
Complotype
