摘要
目的:探讨成年大鼠骨髓间充质干细胞(MSC)向神经细胞体外定向诱导分化的条件。方法:从正常SD大鼠骨髓中分离获取MSC,体外培养扩增纯化后传代于塑料培养皿中,采用丁羟茴醚(BHA)和二甲亚砜(DMSO)对传至3～5代的细胞进行体外诱导分化,采用免疫细胞化学对诱导后的细胞进行鉴定。结果:诱导1 h、2 h、3 h后有部分细胞表达神经干细胞标志蛋白nestin,且随诱导时间延长呈下降趋势;诱导5 h后,大部分细胞具有典型神经元形态,表达神经元标志物-神经元特异性烯醇化酶(NSE),少部分细胞呈星型胶质细胞状,表达胶质细胞标志物-胶质纤维酸性蛋白(GFAP)。结论:骨髓间充质干细胞可以在体外培养扩增并诱导成为神经元样细胞和星型胶质样细胞。
Aim:To investigate the culture and neuronal induction of bone mesenchymal stem cells(MSCs) from adult rats. Methods:The MSCs were isolated primarily from bone marrow of adult rats and purified by passage culture. The 3rd to 5th generation of MSCs were planted into plastic petridishes and induced by butylated hydroxyanisole( BHA) and dimethyl sulfoxide( DMSO). Immunocytochemistry were used to identify cell types. Results: After induction for 1 h, 2 h,and 3 h, some of the MSCs-derived cell expressed nestin, a neural stem cell marker. After induction for 5 h, a large number of cells displayed classical neuron-like morphology, and expresed neuron specific enolase(NSE) ;a small number of cells expressed glail fibrillary acidic protein ( GFAP) which was the astrocytes specific marker. Conclusion: MSCs can be cultured , proliferated and differentiated into neural cells and glial cells in vitro.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2004年第2期269-271,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省医学科技创新人才工程资助项目
河南省高校杰出科研人才创新工程项目
河南省重点科技攻关基金资助项目 01170319
关键词
骨髓间充质干细胞
细胞培养
神经细胞
分化
mesenchymal stem cell
cell culture
neuronal cell
differentiation
