摘要
目的 :观察丹参酮对实验性动脉再狭窄的防治作用 ,并初步探讨其作用机制。方法 :雌性KM小鼠随机分为模型组、高剂量组、低剂量组 ,每组 12只。将小鼠左颈总动脉近动脉分叉处结扎 ,造成内膜增生、颈动脉狭窄模型。将模型组结扎动脉对侧血管作为正常组。术前两天开始给药 ,高、低剂量组分别灌胃给予丹参酮 6,3g·kg- 1·d- 1,模型组给予等体积的溶媒。给药 4周后 ,取颈动脉作HE染色 ,光镜下观察动脉形态并用图像分析系统分析动脉内膜面积、中膜面积、相对管腔面积及内膜 /中膜面积 ,用免疫组化测定增殖细胞核抗原 (PCNA)的表达水平并计算阳性指数。结果 :与正常组比较 ,模型组管腔相对面积明显减小 (P <0 .0 1) ,内膜面积、中膜面积及内膜 /中膜面积增大 ,差异显著 (P <0 .0 1) ;与模型组比较 ,丹参酮低剂及高剂组的管腔相对面积均显著增大 (P <0 .0 1) ,内膜面积、中膜面积及内膜 /中膜面积均有减小 (P <0 .0 5) ,PCNA的表达明显减少 ,阳性指数显著降低 (P <0 .0 1)。结论 :丹参酮可抑制由于血流动力学改变而引起的以平滑肌细胞增殖迁移为主要病理特征的内膜增生 ,提示对经皮腔内冠状动脉血管成形术 (PTCA)
Objective: To observe the preventive and t herapeutic effect of tanshinone (TA) on artery restenosis in mice and primarily explore the mechanism. Method: Female KM mice were random ly divided into model control, low dose and high dose TA groups. Each group had 12 mice. The low and high dose drug groups were respectively given TA 3 and 6 g ·kg -1 ·d -1 by ig; the model control group was given the same volume solvent. The controlateral carotid of ligated artery of model control group was regarded as normal control. 2 days later, the mice's left common carotid art ery was dissected and ligated near the carotid bifurcation, leading to intima hy perplasia and then establishing restenosis model. 4 weeks later, the artery was harvested and stained by hematoxylin-elsin (HE) and immunohistochemistry of PCN A. The morphological changes were checked under microscope; the area of the inti mal and medial layer of the vessels, and their ratios were analyzed with image a nalysis software. The expression level of PCNA was expressed as the positive i ndex. Result: Compared with those of normal artery, the in timal area, media area and intima-to-media ratio of ligated artery increased o bviously (P<0.01). But TA could significantly decrease all of these paramete rs (P<0.01), and also decrease the positive index of PCNA (P<0.01). Conclusion: TA effectively inhibits intima hyperplasia, which is mainly characterized with the proliferation and migration of smooth muscle ce ll induced by abnormal hemodynamic changes. This result suggestes that TA may pl ay a positive role in the prevention of restenosis after PTCA.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2004年第3期255-258,共4页
China Journal of Chinese Materia Medica
