摘要
CDC16Hs是细胞周期末期促进复合物 (APC)的亚基 .利用基于LexA的酵母双杂交系统 ,把它作为诱饵蛋白筛选人胎脑文库 ,发现它与DNA双链断端修复蛋白Ku80的羧基端相互作用 .CDC16Hs和全长Ku80的结合通过 pulldown实验在体外得到验证 .
CDC16Hs is the subunit of anaphase-promoting complex. CDC16Hs was used as bait protein to screen its interactors in the human fetal brain cDNA library in LexA-based yeast two-hybrid system. In the screen, the C-terminal partial of Ku80, a DNA double-stranded breaks repairing protein was identified to interact with CDC16Hs. The in vivo interaction between CDC16Hs and full length of Ku80 was confirmed in the pull down test.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
2004年第2期135-140,共6页
Journal of Fudan University：Natural Science
基金
上海市肿瘤研究所合作项目
新世界集团公司的资助
