摘要
目的 :观察补肾益气活血方含药血清对体外培养的兔关节软骨细胞的增殖凋亡和端粒酶活性的影响。方法 :取兔的关节软骨 ,将软骨细胞分离传代后 ,分为补肾益气活血方含药血清组和对照组 ,置入 37℃、5 0ml/L的CO2 培养箱内培养 7d后 ,采用MTT法检测细胞增殖 ;TUNEL法检测各组软骨细胞的凋亡 ;软骨细胞经扩增后用ELISA检测端粒酶活性。结果 :①补肾益气活血方高、中、低剂量组细胞增殖均高于对照组 (P <0 .0 5 )。②补肾益气活血方含药血清各组的软骨细胞凋亡率明显低于对照组 (P <0 .0 5 )。③补肾益气活血方含药血清各组的软骨细胞端粒酶活性明显高于对照组。结论 :补肾益气活血方可能通过上调软骨细胞端粒酶活性 。
Objective: To observe the effects of kidney supplementing, Qi invigorating and blood activating (KQB) Prescription containing serum on proliferation and apoptosis of rabbit chondrocytes in vitro. Method: The chondrocytes were isolated from the cartilage of the rabbits, and were divided into 5%, 10%, 20% KQB prescription containing serum groups and control group. After the chondrocytes were cultured for 7 days in 37℃,50 ml/L CO 2 incubator, their proliferation, apoptosis and the telomerase activity were detected with MTT, TUNEL and PCR ELISA respectively. Result: The proliferation of chondrocytes in 5%,10% ,20% KQB prescription containing serum groups were respectively higher than those in control group(P<0.05);their apoptosis were respectively lower than those in control group(P<0.05);their telomerase activity were respectively higher than those in control group(P<0.01). Conclusion: KQB Prescription can inhibit the apoptosis of chondrocytes by up regulating the telomerase activity.
出处
《安徽中医学院学报》
CAS
2004年第2期42-44,共3页
Journal of Anhui Traditional Chinese Medical College
关键词
补肾益气活血方
软骨细胞
增殖
凋亡
中药
Kidney supplementing, Qi invigorating and blood activating Prescription
Chondrocytes
Apoptosis
Telomerase
