摘要
①目的 探讨Epstein BarrVirus(EBV)相关胃癌 (EBVaGC)组织中bcl 2和Ki 6 7蛋白表达与EBV感染的关系。②方法 应用PCR Southern技术 ,检测 1 85例胃癌及相应癌旁组织中EBVDNA ,原位杂交技术检测PCR阳性标本EBV小RNA1 (EBER1 )的表达 ,确证EBVaGC。并选择EBV阴性胃癌 (EBVnGC)标本 ,应用免疫组化技术检测其bcl 2和Ki 6 7蛋白的表达。③结果 1 85例胃癌组织中检测到EBVaGC 1 3例 ,阳性率为 7.0 3% ,1 85例癌旁组织均为阴性 ,胃癌组织和相应癌旁组织EBV阳性率比较差异有显著性 (χ2 =1 1 .0 77,P <0 .0 0 1 )。E BVaGC和EBVnGC组织中bcl 2表达率分别为 5 3.85 %和 4 8.89% ,二者比较差异无显著性 (χ2 =0 .0 99,P >0 .0 5 )。EBVaGC和EBVnGC组织中Ki 6 7指数 (KI)平均值分别为 2 8.2 5 4± 6 .2 84和 37.86 4± 1 4 .5 2 3,二者比较差异有极显著意义 (t′=2 .5 5 3,P <0 .0 1 )。④结论 EBV感染与部分胃癌的发生有一定相关性 ,EBVaGC的发生与bcl 2蛋白表达无明显相关性 ,与Ki 6 7蛋白表达有一定相关性。
Objective To study the expression of bcl-2 and Ki-67 proteins in Epstein-Barr virus(EBV)-associated gastric carcinomas(EBVaGC). Methods EBV DNA was tested with PCR-Southern analysis in 185 gastric carcinomas and corresponding paracarcinomas. For PCR positive samples EBV-encoded small RNA 1 (EBER1) was tested to confirm EBVaGC by in situ hybridization(ISH). EBVaGC and EBV-negative gastric carcinomas(EBVnGC) were tested for the expression of bcl-2 and Ki-67 proteins by immunohistochemical method. Results EBVaGC was detected in 13(7.03%) of the 185 gastric carcinoma tissues,but not in the corresponding paracarcinomas. The EBV positivity in carcinomas was significantly different from that in paracarcinomas (χ 2=11.077,P<0.001). The bcl-2 immunoreactivity was detected in seven(53.85%) of 13 EBVaGCs and in 22(48.89%) of 45 EBVnGCs , with no significant difference between them (χ 2=0.099,P>0.05). The mean Ki-67 labeling indices were 28.254± 6.284 and 37.864±14.523 in EBVaGCs and EBVnGCs, respectively, with significant difference between them(t′=2.553, P< 0.01 ). Conclusion EBV infection is associated with part of gastric carcinomas. EBVaGC is associated with the expression of Ki-67 protein, but not with the expression of bcl-2 protein.
出处
《青岛大学医学院学报》
CAS
2004年第1期57-59,共3页
Acta Academiae Medicinae Qingdao Universitatis
关键词
EBV
胃癌
BCL-2
KI-67
蛋白
表达
检测
癌组织
herpesvirus 4, human
stomach neoplasms
in situ hybridization
immunohistochemistry
genes, bcl-2
genes, Ki-67
