期刊文献+

δ、μ和κ型阿片肽受体在人类血管壁组织中的表达 被引量:3

EXPRESSION OF OPIOID(δ,μ,κ )RECEPTORS IN THE WALL OF HUMAN BLOOD VESSELS
下载PDF
导出
摘要 ①目的 检测人类周围血管组织中三种阿片肽受体存在与分布情况 ,探讨其意义。②方法 应用免疫组化技术检测了 6 0例含血管的新鲜组织 (子宫 2 0例 ,肺 1 2例 ,卵巢 1 2例 ,脾 1 0例 ,甲状腺 6例 )冷冻切片中δ型阿片肽受体 (DOR)、μ型阿片肽受体 (MOR)和κ型阿片肽受体 (KOR)的表达情况。以血管壁着棕黄色者判为阳性。③结果 DOR、MOR、KOR的表达阳性率分别为 5 3.3%、5 6 .7%和 6 8.3% ,三者比较差异无显著性 (χ2 =3.0 88,P >0 .0 5 ) ,且DOR、MOR和KOR在不同器官中的表达均无差异 (χ2 =6 .6 1 7~ 7.81 5 ,P >0 .0 5 )。在子宫、卵巢、脾、甲状腺血管内膜及肺泡壁毛细血管均有棕黄色着色 ;在子宫、卵巢和甲状腺可见部分组织细胞亦着色。④结论 KOR、DOR、MOR在人类周围血管组织确有表达 ,并多在血管内膜着色 ;三种亚型受体间阳性表达率无明显差异 ; Objective To detect the existence and distribution of opioid receptors in human peripheral blood vessels. Methods An immunohistochemical (IHC) technique was used to detect KOR, MOR and DOR expressions in 60 human tissues containing blood vessels with 20 from uterus, 12 from lung, 12 from ovary, 10 from spleen, and 6 from thyroid. The appearance of brown color in the blood vessel wall was regarded as positive. Results The positive rates of KOR, MOR and DOR were 68.3% , 56.7% and 53.3%, respectively, without any significant difference between them (χ 2=3.088, P>0.05). The positive rates of the receptors in different tissues showed no significant difference (χ 2=6.617-7.815, P>0.05).The brown color was stained in all vessel walls of the uterus, ovary, spleen, thyroid and lung; part of the tissue cells of the uterus, ovary and thyroid was also stained. Conclusion Opioid receptors do express in the endothelium of human peripheral blood vessels. No significant difference exists in the positive expression of the three receptors. The expression of the same receptor in different tissues shows no evident difference.
出处 《青岛大学医学院学报》 CAS 2004年第1期34-36,共3页 Acta Academiae Medicinae Qingdao Universitatis
关键词 阿片样受体 血管 免疫组织化学 周围血管组织 receptors, opioid blood vessel immunohistochemistry
  • 相关文献

参考文献10

  • 1[1]Balasubramanian S, Ramakrishan S, Charboneau R, et al. Morphine sulfate inhibits ypoxia-reduced vascular endothelial growth factor expression in endothelial cells and cardic myocytes[J].J Mol Cell Cardiol,2001, 33(12):2179. 被引量:1
  • 2[2]Chang SL, Felix B, Jiang Y, et al.Action of endotoxin and morphine[J].Adv Exp Med Boil,2001,493:187. 被引量:1
  • 3[3]Malinnowska B, Godlewski G, Schlicker E.Function of nociceptin and opioid OP4 receptors in the regulation of the cardiovascular system[J].J Physiol Pharmacol, 2002 , 53(3):301. 被引量:1
  • 4吕顺艳,朱妙章,臧益民,陈绍洋.外周内源性阿片肽对心血管功能的调节[J].心功能杂志,1999,11(2):106-108. 被引量:1
  • 5[5]Evans CJ, Keith DE, Jr H, et al. Cloning of a delta opioid receptor by functional expression[J].Science, 1992,258:1952. 被引量:1
  • 6[6]Sciorsci RL,Bianchi P, Mononia P. High levels of endorphin and related pathologies of veterinary concern[J]. Immunopharmacol Immunotoxical,2000,22(4):575. 被引量:1
  • 7孙凤艳,于桂华,张安中.血管中的κ型阿片受体[J].中国药理学报,1983,4(2):100. 被引量:1
  • 8林树新,范谨之,王复周.脑啡肽对离体动脉条收缩活动的影响及其机制分析[J].动物学报,1992,38(2):202-207. 被引量:4
  • 9[9]Baker CH,Sutton ET, Zhou Z, et al. Reduced microvascular adrenergic receptor activity due to opoids in endoxin shock[J]. Circ Shock,1990,32(2):101. 被引量:1
  • 10[10]Wong TM,Lee AY,Tai KK. Effects of drugs interacting with opioid receptors during normal perfusion or ischemia and reperfusion in the isolated rat heart-an attempt to identify cardiac opioid receptor subtype involved in arrhythmogenesis[J]. J Mol Cell Cardiol,1990,22:1167. 被引量:1

二级参考文献10

共引文献3

同被引文献31

引证文献3

二级引证文献11

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部