摘要
目的 :探讨参附注射液治疗肿瘤恶病质的作用机制。方法 :应用T739恶病质动物模型的血细胞和瘤组织提取mR NA ,分别在 2 0S芯片上杂交 ,对差异表达基因进行分析探讨。结果 :血细胞和瘤组织芯片各得到 5 6条差异表达基因 ,分别有5 7 1%和 5 5 4 %是小鼠肌cDNA文库中的基因 ,其中相交叉的基因有 9条 ,均呈同一的上调或下调趋势 ,有 4条是小鼠肌cDNA文库中的基因。讨论 :1.本结果提示肌cDNA文库的基因片段可能引起肌肉组织的过度消耗 ,导致恶病质状态。 2 研究已证实 ,炎性细胞因子与恶病质密切相关 ,ADAMTS 1基因与炎症的进程相关 ,本实验显示该基因呈上调趋势 ,值得深入研究 ;3 SCD2在本实验中呈上调趋势 ,推测其表达与脂肪组织的代谢与功能相关。 4 .本实验中FKBP5 1显著下调 ,与同期进行的甲孕酮抗恶病质基因芯片实验得到的趋势相同 ,提示FKBP5 1在调节恶病质发生发展方面有与之相近的作用。 5 .PTA是胸腺激素家族的成员 ,该基因表达为显著上调。提示了参附注射液在治疗恶病质状态的免疫调节作用。
Purpose:To explore the mechanism of Shenfu Injection in treating cancer cachexia (C.C.) Method:mRNA was extracted from the blood cells and tumor tissue of T739 animal model of C.C., hybridizated respectively on 20S gene chip.Analysis on differential expression genes was carried out.Result:56 differential expression genes were obtained from each chip of blood cell and tumor tissue,57.1% and 55.4% of which belongs to mouse musculus cDNA library. 9 cross-genes were obtained from each chip of blood cell and tumor tissue,showing the same tendency of up-regulation or down-regulation. 4 of them belongs to mouse musculus cDNA library.Disscussion:1.The result suggests The gene segment of mouse musulus cDNA library might cause over-consumption of muscular tissue and results in C.C.; 2.Previous studies confirmed that inflammatory cytokines,such as IL-1 and IL-6 are closely related to C.C. Gene expression of ADAMTS-1 is correlated with inflammatory proceeding.The result showed that ADAMTS-1 of cross-genes showed a tendency of up-regulation,which is worth further studying; 3.The gene SCD2 showed a tendency of up-regulation. It was inferred that its expression was correlated with metabolism and function of adipose tissue,which further affected malnutrition in C.C. stage; 4.Described as progesterone receptor-associated immunophilin,FKBP51 displays interactions with progesterone receptor.The gene showed the same tendency as the gene chip study of medroxyprogesterone anti-C.C. at the same stage. As medroxyprogesterone was a confirmed effective drug for C.C., FKBP51 was deduced to have the same function as medroxyprogesterone. 5.PTA is the member of thymosin family and has immune regulatory effect.The gene expression showed a tendency of up-regulation after Shenfu Injection treatment.Shenfu Injection was thought to stimulate T cell immunity and further improve symptoms through elevating immune ability.
出处
《中国实验方剂学杂志》
CAS
2004年第2期22-26,共5页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
参附注射液
恶病质
基因组学
Shenfu Injection
cancer cachexia
genomics
