摘要
本文通过分子杂交技术,以恶性疟原虫为靶DNA,对从恶性疟基因文库中筛出的含有高度重复顺序的pBF_4DNA探针和含单考贝基因的pBF_(13)DNA探针以及恶性疟原虫全基因组DNA探针进行了杂交比较,结果pBF_(13)DNA探针比全基因组DNA探针敏感度低10倍,比pBF_4DNA探针敏感度低100倍,全基因组DNA探针可检到的DNA含量为100pg。
To compare the sensitivity of DNA probe in detection of Plasmodium falciparum, three different DNA probes were prepared. P. falciparum genomic DNA was prepared from cultured parasite, pBF_(13) DNA was produced from the clone which was selected from P. falciparum genomic DNA library containing single copy gene, and pBF_4 DNA was produced from the clone containing highly repeated gene sequence. The 3 kinds of DNA probes were labelled with^(32)P and hybridized with P. falciparum DNA on nitrocellulose paper at the same condition. The results showed that the sensitivity of pBF_(13) probe was 10 times less than the whole genomic DNA probe and pBF_4 DNA probe was 10 times more sensitive than the whole genomic DNA probe. The whole genomic DNA probe could detect 100pg DNA of the parasite.
出处
《中国寄生虫病防治杂志》
CSCD
1992年第2期96-98,共3页
Chinese Journal of Parasitic Disease Control
