摘要
目的:探讨饮食中铁过载诱导肝组织纤维化形成的组织学变化. 方法:采用(?)SD大鼠,在饮食中适量添加铁,共饲喂9 wk, 然后处死动物,收取肝组织,分别在石蜡切片和透射电镜下观察肝组织损害、肝窦毛细血管化和窦周纤维化形成以及I、Ⅳ型胶原和Laminin的分布情况. 结果:铁剂组大鼠可见肝细胞凝固坏死,星状细胞活化, 肝窦毛细血管化和轻微窦周纤维化,I型胶原和laminin含量增多,中央静脉基底膜增厚. 结论:饮食中铁过载持续一定时间可损害肝细胞,激活肝星状细胞,减少窦内皮细胞的窗孔,导致肝窦毛细血管化和窦周肝纤维化,故酒精性肝病造模可考虑配合使用.
AIM: To study the histological changes of hepatic fibrosis induced by diet iron overload in SD rats. METHODS: After living on an iron overload in diet for 63 days, the male SD rats were decollated and the livers were harvested to examine the liver indexes, morphology of hepatocytes, hepatic stellate cells (HSC), sinusoidal endot-helial cells, sinusoid, peri-sinusoid, and distribution of collagen type Ⅲ and laminin were also observed. RESULTS: On model rats there were coagulation necrosis of hepatocytes, activated HSC, sinusoidal endothelial cells with less fenestrae, sinusoidal capillarization and light peri-sinusoid fibrosis, thickened basement of central vein, more collagen types Ⅰ and Ⅱ and laminin than those in normal controls. CONCLUSION: Iron overload in diet for some time can injure hepatocytes, activate HSC, decrease the fenestrae of sinusoidal endothelial cell, and result in sinusoidal capillarization and peri-sinusoid fibrosis. It may be feasible to establish an ALD model with iron overload in diet.
出处
《世界华人消化杂志》
CAS
2004年第3期714-718,共5页
World Chinese Journal of Digestology
