摘要
以黄喉拟水龟 (Mauremysmutica)的红血细胞为材料 ,以鸡红血细胞为DNA标准 ( 2 5pg/2c) ,采用流式细胞仪测定了黄喉拟水龟及其两个种群的细胞核基因组DNA含量。黄喉拟水龟的细胞基因组DNA含量为 5 16± 0 2 9pg/2c (n =6 0 ) ;南方种群的细胞核DNA含量为 5 19± 0 30pg/2c (n =30 ) ,北方种群为 5 14±0 30pg/2c (n =30 ) ,两个种群的核DNA含量无显著差异 (t=0 6 84 7,df =5 8,P >0 0 5 )。
The cellular DNA contents of the yellow pond turtle ( Mauremys mutica ) and its two populations were studied by using a flow cytometer with the DNA of chicken red blood cells (2 5 pg/2c) as a standard.The DNA content of the turtle is 5 16±0 29 pg/2c ( n =60).The DNA content of southern population is 5 19±0 30 pg/2c ( n =30) and that of northern population is 5 14±0 30 pg /2c ( n =30),and there is no difference between southern and northern populations of M.mutica ( t =0 6847, df =58, P >0 05).
基金
广东省海洋与渔业局重点项目资助 (2 0 0 1A0 9)
中国科学院淡水生态与生物技术国家重点实验室开放课题基金项目资助(KSCX2 -SW -3 0 3 )
