摘要
以白洛克肉鸡 (EE)、中国丝羽乌骨鸡 (CC)、农大褐 (DD)和白来航 (AA) 4个纯种鸡为材料 ,进行 4× 4完全双列杂交 ,共得到 16种杂交组合。应用mRNA差异显示技术 (DDRT PCR)检测了 8周龄纯种和杂种鸡之间肝脏组织基因的差异表达。结果表明 ,在纯种和杂种间共有 8种 15类基因差异表达模式 ,杂种和纯种之间基因表达存在明显的差异。对各种基因差异表达模式与 10个肉用性状的杂种优势率进行相关分析发现 ,表达一致型P8(t1111)与肉用性状的杂种优势率相关不显著 (P >0 0 5) ,这说明杂种优势的形成与某些基因的差异表达有关 ;正交或反交特异表达型P4(t0 10 0、t0 0 10 )与 8周龄个体重、腿肌重、半净膛重、全净膛重相关显著 (P <0 0 5) ,与胸肌重相关极显著 (P <0 0 1) ;单亲特异表达型P1(t10 0 0、t0 0 0 1)与腹脂重相关显著 (P <0 0 5) ,与体斜长相关极显著 (P <0 0 1) ;双亲特异表达型P7(t10 0 1)与腿肌重、翅重、半净膛重、肌间脂宽的杂种优势率相关显著 (P <0 0 5) ;正交或反交单亲表达一致型P2 (t110 0、t0 0 11、t10 10、t0 10 1)与肌间脂宽的杂种优势率相关显著 (P <0 0 5) ;单亲表达一致型P5(t1110、t0 111)胫骨长的杂种优势率相关显著 (P <0 0 5)。
The concept of heterosis has already been put forward for a century.The hypothesis of Dominance,Superdominace and Epistasis has also been brought forward to explain the phenomenon of heterosis.As we know,there is spatio-temporal speciality about the expression of gene and only expressed genes contribute to the formation of heterosis.So the study on heterosis in expression level becomes more meaningful.A lot of studies on heterosis in this level have been done in plants,but there is no such study carried on animals in this area.In this study,the technique of mRNA Reverse Transcription Differential Display was used to research the heterosis molecular mechanism of animal.In order to expound the molecular genetic mechanism of animals heterosis,the 4×4 completely diallele cross experiment of 4 purebreds chicken was conducted among White Polymouth Rock (EE),Chinese Silk Chicken (CC),CAU Brown (DD) and White Leghorn (AA).The chicken of 16 cross combinations were reared to 8 weeks old,then 30 chicken in each combination were selected randomly and slaughtered.The traits of body weight of 8 weeks,wing weight,eviscerated weight,eviscerated weight with giblet,breast muscle yield,leg muscle yield,body length,abdomen fat weight,interamuscular fat width,tibia length were measured,and in which 8 individuals in each combination were selected randomly to collect the liver tissue samples,which were stored in liquid nitrogen or at -80℃ to be used for total RNA (TRNA) extracting.After the total RNA(TRNA) was extracted,16 TRNA pools were formed in the same quantitative according to the concentration of 8 individual TRNA.They were reversely transcribed with three anchor primers H-T_11A,H-T_11G and H-T_11C.Then the reverse transcription PCR for each transcript product was done in two repeats at the same time with the same anchor primers and 8 random primers.The polyacrylamide gel electrophoresis of each PCR product was run in Bio-Rad Power 3 000 temperature control system.After electrophoresis,the gel was stained by AgNO_3 according
基金
国家重点基础研究发展规划项目(批准号 :TG2 0 0 0 0 1 6 1 0 5)资助~~
关键词
鸡
杂种优势
MRNA差异显示
基因差异表达模式
chicken
heterosis
mRNA differential display
gene differential expression patterns
