摘要
壳聚糖经α-淀粉酶水解并用乙醇溶液纯化可得到不同相对分子质量的壳聚糖。用重均分子量为20ku和1ku的壳聚糖处理黄瓜种子和幼苗可以诱导黄瓜植株产生过氧化物酶和β-1,3-葡聚糖酶。壳聚糖的这种诱导效应对剂量和相对分子质量都有依赖,并有一定的滞后期。壳聚糖对黄瓜菌核病的抗病性随喷施次数增加而增加。在对黄瓜种子进行浸种处理时,重均分子量为20ku的壳聚糖诱导效果更为明显;在对黄瓜叶片喷洒处理时,1ku的壳聚糖的诱导效应更为明显。
Chitosan hydrolyzates with different molecular weights released enzymatically were shown to induce plant activity. Peroxidase and β-1,3-glucanase were over-expressed for defense response when they were used to treat cucumber plants. Such effects of hydrolyzates were dose-dependent and molecular weight-dependent, and had to undergo a lag phase. With the increase of spraying times the anti-disease (ratio) to Sclerotinia sclerotiorum increased. The inducing effects of chitosan with molecular weight of 20 ku were more obvious when cucumber seed soaked in chitosan solution. However, chitosan with molecular weight of 1 ku showed better induce effect when sprayed on cucumber leaves.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第2期148-152,共5页
Journal of East China University of Science and Technology
基金
国家863项目(2002AA90240)
