摘要
本工作在离体灌流的大鼠心脏缺血-再灌注（I/R）损伤模型上,观察了脂质体携载L-精氨酸（L-Arg）的保护作用。结果发现,1mol/L L-Arg脂质体灌流心脏,明显抑制缺血心脏再灌注时心肌MDA含量和线粒体Ca<sup>2+</sup>含量的增加,显著减少心肌细胞内的酶（乳酸脱氢酶、组织强白酶D）和肌红蛋白的漏出及心肌MDA的释放,明显增加冠脉流量。而相同剂量的游离L-Arg则无明显保护效应。我们在离体大鼠灌流的主动脉条上进一步发现,L-Arg脂质体具有显著的血管舒张效应。而游离L-Arg则不具有明显舒张效应。结果表明,脂质体作为药物载体,包裹内源性EDRF释放剂防治心肌I/R损伤是有效的。可能具有临床应用前景。
The article reported the protective effects of liposome-carried L-arginine (L-Arg) against ischemia-reperfusion (I/R) damage in isolated perfused rat hearts. It was found that perfusion of ischemic heart with 1 mmol/L L-Arg-liposome significantly inhibited the reperfusion-induced increasing of myocardial MDA and mitochondrial calcium, markedly alleviated leakage of intracellular enzyme (lactate dehydrogenase, cathepsin D) and release of myocardial MDA, and elevated coronary perfusion flow dramatically. How ever, perfusion with free L-Arg at the same dosage (1 mmol/L) had no protective effect. Furthermore, L-Arg-liPosome had significant vasodilation action on the isolated perfused rat aortic strips, but not with free L-Arg. These results suggested that liposome as drugcarrier, entrapping precursor of EDRF is more effective for prevention and therapy of myocardial I/R injury, and might be promising in future clinic practice.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1992年第3期273-276,共4页
Chinese Journal of Pathophysiology
关键词
精氨酸
脂质体
心肌
灌注法
Arginine
Liposomes
Myocardium
Perfusion
