摘要
目的 :探讨藤黄酸 (gambogicacid ,GA)诱导体外培养人胃腺癌SGC 790 1细胞凋亡的作用。方法 :通过MTT比色法分析藤黄酸对人胃癌SGC 790 1细胞在 2 4 ,4 8,72h增殖的影响 ,并通过光镜、电镜观察细胞形态学的改变及流式细胞术检测藤黄酸对SGC 790 1肿瘤细胞周期动力学和凋亡诱导率的影响 ,用免疫组化方法检测凋亡相关基因bax和bcl 2的蛋白表达变化。结果 :藤黄酸可明显抑制SGC 790 1细胞的增殖 ,4 8h半数生长抑制剂量 (IC50 )为 1.4 7,1.6 μmol/L的藤黄酸可导致 790 1细胞形态学的改变 ,流式细胞术显示细胞周期亦发生变化 ,G2 /M期细胞增加 ,凋亡率呈时间 剂量相关性。同剂量的藤黄酸还可增加抑癌基因bax和减少诱癌基因bcl 2的蛋白表达量。结论 :藤黄酸可明显抑制SGC 790 1肿瘤细胞的生长 ,诱导细胞凋亡 ,其分子机制可能与其减少Bcl 2 /Bax的比值相关。
AIM: To study the inducing apoptosis of Gambogic Acid (GA) on human gastric adenocarcinom SGC-7901 cells. METHOD: Inhibition of proliferation(24,48,72 h)was measured with MTT (3-[4,5-dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. The marked morphological changes were confirmed by microscopy. A great quantity of apoptotic cells and the increasing G_ 2 /M phase cells were observed by flow cytometry. The expression of bax and bcl-2 genes were detected by immunohistochemistry.RESULT: GA has obvious inhibition on the growth of SGC-7901 cells. The value of IC_ 50 for 48 h was 1.47 μmol/L. After treatment of SGC-7901 cells with GA 1.6 μmol/L,marked morphological changes and the increased apoptosis cells were observed. GA can also increase the proportion of G_ 2 /M phrase cells. The expressions of bax gene increased and bc1-2 gene decreased. The activation of bax and the suppression of bc1-2 may contribute to the apoptosis mechanism. CONCLUSION: GA inhibited proliferation and induced apoptosis of SGC-7901 cells.
出处
《中国天然药物》
SCIE
CAS
CSCD
2004年第2期106-110,共5页
基金
国家高新技术研究发展计划 (863项目 ,No .2 0 0 2AA2Z3 112 )
江苏省高技术产业化促进中心基金 (No .0 10 40 12 )
江苏省科技攻关项目 (No .BE2 0 0 1618)基金资助~~
