摘要
目的探讨低剂量T鄄2毒素(<100μg/kg)对机体生化指标的影响。方法选用Wistar大鼠30只,在饲料中按10、50、100μg/kg添加T鄄2毒素,5周后,测定血清中丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH鄄Px)、腺苷脱氨酶(ADA)、羟脯氨酸(HYP)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、γ鄄谷氨酰转肽酶(γ鄄GT)、肌酸激酶(CK)、α鄄羟丁酸脱氢酶(HBDH)、乳酸脱氢酶(LDH),并加以比较分析。结果除GSH鄄Px、HYP随给毒剂量的增加而下降,γ鄄GT改变不明显外,其余7项指标均随给毒剂量增加而上升。其中有统计学意义的有MDA(P<0.01)、GSH鄄Px(P<0.01)、HYP(P<0.05)、AST(P<0.05)、LDH(P<0.05)。结论低剂量T鄄2毒素可致机体多种生化指标改变,提示低剂量T鄄2毒素可能引起机体多器官损伤。
Objective To examine the effect of minidose (< 100 μg/kg) T-2 toxin on rat. Methods Thirty Wistar rats were divided into four groups, the experimental groups were fed fodder with T-2 toxin of 10 μg/kg, 50 μg/kg, and 100 μg/kg, respectively for 5 weeks. Reagent kits and biochemical auto-analysis apparatus were used to detect the biochemical indexes in serum. Results The content of GHS-Px and HYP were reduced with the increase of T-2 toxin, that of γ-GT had no obvious change, and the other indexes increased with the increase of T-2 toxin, in which MDA (P < 0.01), GSH-Px (P < 0.01), HYP(P < 0.05), AST (P < 0.05) and LDH (P < 0.05) had statistic significance. Conclusion T-2 toxin of minidoses can cause many biochemical indexes change, these results show that T-2 toxin of minidoses can cause damage in many organs of rat.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2004年第2期116-118,共3页
Chinese Jouranl of Endemiology
基金
黑龙江省杰出青年课题基金资助项目(J01鄄04)
