摘要
目的 :探讨成年大鼠骨髓间充质干细胞 (MSCs)的体外培养和向神经元样细胞分化的能力。方法 :利用Percoll分离液 (1.0 73× 10 3 g/L)梯度分离成年大鼠MSCs,采用 3种不同的方法对其诱导分化 ,免疫细胞化学方法检测神经元特异性烯醇化酶 (NSE)、神经丝蛋白 (NF)、星形胶质细胞特异性标志 (GFAP)和神经前体细胞(Nestin)的表达。结果 :成功进行了成年大鼠MSCs的原代和传代培养 ,3种诱导方法均能使MSCs分化为神经元样细胞 ,都能表达NSE、NF和Nestin ,而不表达GFAP。结论 :MSCs能在体外分离、扩增、传代 ,并能向非间充类细胞分化。
Objective: To study the isolation, purification, and differentiation of mesenchymal stem cells(MSCs) into nerve cells in rats in vitro. Methods: Bone marrow mononuclear cells from femur and tibia of rats were obtained sterilely with gradient centrifugation by using Percoll (1.073×103 g/L ). The low density cells including MSCs were cultivated and induced to differentiate into nerve cells with 3 different treatment protocols. Neuron-specific enolase (NSE), neurofilament (NF), glial fibrillary acidic protein (GFAP), and Nestin were detected by using immunocytochemistry method, and quantitation analysis of neuronal differentiation. Results: The MSCs were successfully cultivated primarily and secondly. All 3 treatment protocols induced MSCs to differentiate into neuron-like cells. The neuron-like cells expressed NSE, NF, and Nestin,but could not express GFAP. Conclusion: The MSCs can be cultured and passaged in vitro and induced to differentiate into non-mesenchymal cells.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2004年第2期121-122,132,共3页
Journal of China Medical University
基金
辽宁省博士启动基金资助项目 ( 2 0 0 2 10 3 1)
关键词
骨髓
细胞培养
间充质干细胞
诱导分化
神经元样细胞
bone marrow
cell culture
induction differentiation
neuron-like cells
mesenchymal stem cells
