摘要
生长在含2%甘氨酸的G培养基上的藤仓赤霉菌菌丝体,经二硫苏糖醇作预处理,其细胞壁对纤维素酶和溶菌酶的混合酶液敏感,它们的适宜浓度是纤维素酶1.5%,溶菌酶0.7%。在高渗液中酶解3—4小时,细胞壁被逐渐溶解并大量释放原生质体(约10~7/ml),再生率在50%以上。观察了在PDS液体培养基中原生质体再生成菌丝体的方式,看到菌丝再生或者是单球直接生长或者是经过一次、两次或多次的细胞分裂。对原生质体进行X射线照射诱变和进行终代谢产物高抗性变种筛选,挑高产赤霉素菌株,获得了良好的结果。
Mycelia grown in G medium containing 2% glycine,pretreated with di- thiothretol,was susceptible to mixed enzymes consisting of cellulase and lysozyme,their proper concentrations were cellulase 1.5% and lysozyme 0.7%.After enzymololysis in hypertonic solu- tion at 28℃ for 3—4 h the cell wall was lysed gradually and plenty of protoplasts was relea- sed (about 10~7/ml).Regeneration frequency was over 50%.The regeneration fashion in liquid medium was observed.It showed that mycelia regenerated was by way of growing hyphae from protoplast directly or via once,twice,or multiple cell divisions. The strains producing more gibberellins were screened by X-ray radiation and high con- centration of GA_3 resistant mutation of the prottoplast preprations.
出处
《真菌学报》
CSCD
北大核心
1992年第3期221-228,共8页
基金
上海自然科学发展基金
关键词
赤霉素
藤仓赤霉
原生质体
再生
Gibberellin
Gibberella fujikuroi
protoplast
regeneration
