摘要
【目的】探讨通腑益气法及所组方药对全身炎症反应的调控及防治多器官功能障碍(MODS)的作用。【方法】SD大鼠80只,随机分为空白对照组、模型组、中药低剂量治疗组和中药高剂量治疗组;造模前1d高、低剂量组分别按60g/kg、30g/kg剂量连续给药2次,空白及模型组给予等容量生理盐水;给药后第2天除空白组外其他3组参照文献方法造模,12h后取血清测肿瘤坏死因子α(TNF-α)及一氧化氮(NO)含量,并对主要脏器进行组织病理学检查。【结果】模型组TNF-α与NO水平升高,与空白组比较有显著性差异(P<0.05);中药高、低剂量组能降低血清TNF-α与NO水平,与模型组比较有显著性差异(P<0.05),但未显示明显量效关系。【结论】通腑益气法所组方药可能是通过降低TNF-α与NO水平,抑制全身炎症反应,进而产生防治MODS的作用。
[Objective] To discuss the effect of intestine-dredging and Qi-benefiting method and its corresponding prescription in managing systemic inflammatory response and preventing multiple organ dysfunction syndrome (MODS). [Methods] Eighty. SD rats were randomized to control, model, low-dosage (LD) and high-dosage (HD) groups. One day before modeling, HD and LD groups were administered the preparation based on intestine-dredging and Qi-benefiting method by 60g/kg and 30 g/kg for 2 times, and control and model groups were given equal amounts of normal saline. On the 2nd day after the administration, 3 groups except the control group were modeled on the given reference. Twelve hours after modeling, the content of TNF-α and NO in serum was examined and histopathological examination of the main organs was conducted. [Results] Compared with the control group, the level of TNF-α and NO significantly increased (P<0.05) in the model group, and compared with the model group, greatly decreased in LD and HD groups (P<0.05). But there is no obvious dose-effect relationship. [Conclusion] The preparation based on the intestine-dredging and Qi-benefiting method may inhibit systemic inflammatory response and prevent MODS.
出处
《广州中医药大学学报》
CAS
2004年第2期148-150,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家中医药管理局项目(编号:225)
关键词
通腑益气法
多器官功能障碍
全身炎症反应
调控
MULTIPLE ORGAN FAILURE/TCD therapy
TUMOR NECROSIS FACTOR/blood
NITRIC OXIDE/blood
DISEASE MODELS, ANIMAL
RATS
