摘要
采用改良的微囊藻毒素提取、制备方法可获得一定纯度的MC。经HPLC分析 ,MC RR的含量在 95 %以上。用微囊藻毒素处理细长聚球藻 ,发现毒素能显著抑制聚球藻的生长 ,降低聚球藻的可溶性蛋白与可溶性、不可溶碳水化合物含量 ,改变PC/Chl比值 ,抑制光合系统PSⅡ活性 ,进而导致光合作用减弱 ,生化反应减慢 ,从而抑制该藻的细胞分裂 。
In China, lake eutrophication is serious. Many lakes are close to death because receiving too big amounts of polluted water. Recently, heavy blooms frequently occured in Dianchi Lake and Microcystis is the dominant species in the bloom, and in many cases, when appeared in the lake, forms a continuous bloom all along the year, eliminating quite all the other species. Microcystis produces a family of related cyclic hepatopeptides(microcystins,MC). These toxins are severely hepatotoxic, produced in Microcystis cells and released into water body when algal cells were broken. Most investigations about the toxicity of the microcystins are focused on animals and higher plants. To our knowledge, few studies have been made on the possible effects of microcystins on algae, which are equally important as primary producers in the whole ecosystem. In this study, We chose the unicellular Synechococcus elongatus(one of the most studied, and geographically most distributed, cyanobacteria in the picoplankton) as test material and investigated the toxicological effects of MC-RR on it. For this purpose, some physio-biochemical parameters (cell optical density, chlorophyll fluorescence(Fv/Fm), pigment content(chlorophyll?phycocyanin, soluable protein content, soluable and insoluable carbohydrate content) were tested in algal cells when exposed to 100μg/L microcystin-RR. Microcstin-RR was isolated and purified with the high performance liquid chromatography(HPLC) method. Thereafter, known amount of purified toxin in distilled water was added to test culture flasks in the way to obtain a final toxin concentration in cultures of 100μg·L -1. The results showed that the growth of Synechococcus elongatus(shown as optical density) was significantly inhibited compared with the controls. After exposure to the toxin, the OD 665 were lower than those of the controls from day 2 to day 10. The OD 665 of the control kept in a increasing tendency but that of the toxin -treated decreased on day 8, which meant that the algae h
出处
《水生生物学报》
CAS
CSCD
北大核心
2004年第2期155-158,共4页
Acta Hydrobiologica Sinica
基金
973项目 (2 0 0 2CB412 3 0 0 )
中国科学院知识创新工程重大项目 (KZCX1 SW 12 )
国家重大环境课题"滇池蓝藻水华污染控制技术研究"(K99 0 5 3 5 0 1)
中国科学院方向性创新课题 (2 2 0 3 16)资助
