期刊文献+

大肠杆菌987 P基因工程菌苗预防实验性仔猪黄痢试验 被引量:2

Prevention of experimental neonatal colibacillosis with E.coli 987P genetic engineered vaccine.
下载PDF
导出
摘要 用基因工程技术构建的大肠杆菌HB101/pBP52菌株能产生987 P型菌毛,但不产生热稳定肠毒素(ST),用该茵株制备灭活苗,在产前21天免疫18头第一胎怀孕母猪.在分娩后24~48小时内,用产热稳定肠毒素(ST)大肠杆菌987菌株(O9:K103,987P:NM)人工感染所产仔猪102头,88头仔猪在观察期(攻毒后7天)结束时安全无恙,保护率达86.27%.结果表明:用无毒大肠杆菌HB 101/pBP 52菌株制备的灭活苗可以有效地预防987P菌毛型致病性大肠杆菌引起的仔猪黄痢. Genetic engineered Escherichia coli HB 101/pBP52 strain could produce 987P pili.yet it could not produce heatstable toxin (ST). 18 nulliparous pregnant sows were inoculated with the inactivated vaccine prepared from this strain 21 days before parturition. In 24-48 hours after parturition, 102 heads of their piglets were artificially challenged with ST-producing E. coli 987(O9 : K103 ,987P : NM) ,88 of which were free from any abnormal manifestions within 7 days after challenge with a protection rate of 86. 27%. The results showed that the vaccine made from the strain HB 101/pBP52 could effectively protect piglets from scouring caused by ETEC strain with 987 P pili.
出处 《浙江农业大学学报》 CSCD 1992年第4期54-58,共5页
基金 国家开放实验室资助
关键词 猪病 黄痢 大肠杆菌 疫苗 预防 E. coli vaccine 987 P pili neonatal colibacillosis
  • 引文网络
  • 相关文献

参考文献7

  • 1丁荣法,刘炳祥,胡兰贞,于涟.二元杂交新母猪集中发情方法初探[J].浙江畜牧兽医,1989,0(3):11-12. 被引量:1
  • 2于涟,俞咀英,陈建熹,郑小群,蔡渭明,陈星(鸟旬),余世福,刘军元,赵茶珍,黄顺泉,曹银木.抗原工程苗预防仔猪黄痢试验(第二报)[J]浙江畜牧兽医,1987(03). 被引量:1
  • 3于涟,李雪梅,余新华,费清友.抗原工程苗预防仔猪黄痢试验[J]浙江畜牧兽医,1986(04). 被引量:1
  • 4郭景煜,陈慧珠.大肠艾希氏杆菌987p菌株的初步探讨[J]中国兽医杂志,1983(07). 被引量:1
  • 5郭景煜,陈向经,陈慧珠,潘松年.大肠艾希氏杆菌K_(99)抗原菌株的分析鉴定[J].中国兽医杂志,1981,14(11):2-3. 被引量:9
  • 6郭景煜,陈慧珠,陈向径.抗原菌株的分离[J]中国兽医杂志,1980(10). 被引量:1
  • 7于恩庶等主编..中国人兽共患病学[M].福州:福建科学技术出版社,1996:1266.

共引文献8

同被引文献55

引证文献2

二级引证文献12

;
使用帮助 返回顶部