摘要
目的:了解PER-1型超广谱β内酰胺酶(ESBLs)在革兰阴性杆菌中的流行情况。方法:采用纸片扩散试验初筛和确证ESBLs表型,用聚合酶链反应(PCR)扩增耐药基因及PCR产物测序验证PER-1型ESBLs。结果:ESBLs的总检出率27.3％(179/656),PER-1型占ESBLs的8.9％(16/179),产酶菌以鲍曼不动杆菌为主。结论:应加强对PER-1型ESBLs的检测,以有效控制由产生该型ESBLs细菌引起的医院感染。
Objective: To investigate the prevalence of PER-1 type extended-spectrum β-lactamases (ESBLs) in Gram-negative bacilli. Methods: The phenotypes of ESBLs producing strains were screened and confirmed by disk diffusion test. The resistance genes were amplified by polymerase chain reaction (PCR), and sequential analysis of the PCR products were done to identify the PER-1 ESBL. Results: ESBLs were detected in 27. 3% (179/656) of strains of Gram-negative bacilli. PER-1 gene was found in 8. 9% (16/179) of the ESBLs producing strains. Conclusions:Detection of PER-1 type ESBL among Gram-negative bacilli is important for the control of nosocomial infections caused by these strains.
出处
《中国抗感染化疗杂志》
2004年第1期18-20,共3页
Chinese Journal of Infection and Chemotherapy
