摘要
目的 :观察同型半胱氨酸 ( Hcy)对血管平滑肌细胞 ( VSMCs)胶原合成的影响 ,并探讨其作用机制。方法 :本实验采用体外培养的大鼠 VSMCs,加入不同浓度的 Hcy,通过 3 H-脯氨酸掺入、α1[ ]型胶原测定观察 Hcy对 VSMCs胶原合成的影响 ,并通过半定量 RT- PCR检测其对 α1[ ]型胶原 m RNA表达的影响。结果 :Hcy促进 VSMCs的胶原合成及 α1[ ]型胶原 m RNA表达 ,并呈剂量依赖效应。结论 :Hcy促进 VSMCs的胶原合成 ,可能是通过促进 α1[ ]型胶原 m RNA表达实现的。提示
Objective: To study the effect of homocysteine(Hcy)on collagen synthesis and expression of type Ⅰ collagen mRNA of vascular smooth muscle cells(VSMCs) in culture. Methods: To culture VSMCs of rat with different amount of Hcy in vitro. 3H proline incorporation and type Ⅰ collagen measurement and semi quantitative RT PCR were performed. Results: Hcy enhanced collagen synthesis and upregulated expression of type Ⅰcollagen mRNA of VSMCs in a dose dependent manner. Conclusion: Hcy may promote collagen synthesis through upregulating type Ⅰ collagen mRNA expression in VSMCs in culture. These data indicated that enhancement collagen synthesis may be one of the mechanisms of the pathogenesis of atherosclerosis induced by Hcy.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2003年第2期142-144,共3页
Journal of Jilin University：Medicine Edition
基金
国家教育部留学归国人员启动资金 (1999年 36 3号 )
关键词
半胱氨酸
血管平滑肌细胞
胶原
Cysteine
Vascular smooth muscle cells
Collagen
