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ELISA法间接检测囊虫患者血清特异抗原的探讨 被引量:1

Study on Indirect Detection of Specific Antigen Sera by ELISA in the Cysticercosis Cases
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摘要 目的 探讨简便的检测囊虫抗原的方法。方法 囊液抗原包被酶标板 ,ELISA法检测待检血清与含ELISA法 1∶8(+)的囊虫抗体兔血清的混合液的抗体 ,滴度低于 1∶2 (# )为阳性。结果 囊虫患者阳性率在 82 6 9% - 85 37%之间。讨论囊液抗原间接检测囊虫患者血清特异抗原结果稳定 ,方法简便 ,临床符合率较高。 Aim To find a convenient method to detect cysticercus-antigen.Method Plate coated by antigen of hydatid fluid,blood sera of patients were mixed with the 1∶8(+)(by ELISA)Rabbit blood sera containing cysticercus-antibody,the mixture was detected by ELISA method and blood sample with titre low than 1:2(#)was defined as the positive result.Result The positive rate of t patients was 82.69%-85.37%.Conclusion This method that indirectly detecting specific blood antigen of patients with cysticercosis is proved to be stable?convenient and highly coincidence with clinic.
作者 向莉
机构地区 河南省人民医院
出处 《中国人兽共患病杂志》 CSCD 北大核心 2003年第5期105-105,109,共2页 Chinese Journal of Zoonoses
关键词 ELISA法 囊虫 血清 特异抗原 间接检测 Cysticercus-antigen Indirect detection ELISA
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