摘要
目的 :观察新生犬外周血单个核细胞心肌内移植对急性心肌梗死 (心梗 )心脏功能、左心室血流动力学及左室心梗面积等的影响。方法 :取出生当天新生犬外周血 ,经分离纯化单个核细胞后直接注射进入成年犬前壁心梗区域 ,对照组注射无血清培养基。分别于术中及术后 30 d用 8道生理仪测左室压力、收缩期压力上升最大积分 dp/ dtmax和左室做功量。超声心动图(2 .5 MHz)分别观察术中、术后 30 d心脏功能变化。术后 30 d测量心梗面积、毛细血管密度及心肌宽度。 结果 :术中两组均出现左室压力下降 ,各项指标无明显差别。术后 30 d,细胞移植组心肌内毛细血管密度较对照组高 (P<0 .0 5 ) ,梗死面积缩小(P<0 .0 5 ) ,心肌宽度小 (P<0 .0 5 ) ,左室压力明显高于对照组 (P<0 .0 5 ) ,左室收缩舒张功能明显改善。 结论 :单个核细胞移植可以通过某种机制增加心肌内毛细血管密度 ,缩小梗死面积 。
Objective:To investigate the effect of neonatal dog peripheral mononuclear cells on left ventricular function, haemodynamic, and infarcted size when transplanted into infarcted myocardium. Methods: Mononuclear cells from neonatal dogs were isolated and purified. The descending coronary arteries of adult dogs were ligated and the cells were injected into the border zone of the infarcted region. The control group was injected with non serum culture medium. Left ventricular pressure, d p /d t max , and power of LV were measured during and 30 d after operation. Echocardiogram(HP Sonos 5500, 2.5 MHz) were performed promptly and 30 d after operation to determine the changes of left ventricular function. Infarcted size, capillary density and cardiac muscle width were measured 30 d after operation. Results: LV pressure decreased in both groups, there were no marked differences between the 2 groups. LV pressure and d p /d t max of transplant group were greater than those of control group 30 d after operation. The systolic and diastolic functions were preserved after mononuclear cell transplantation. Infarcted sizes were lower and capillary density was greater in transplant group than those of control group. Conclusion: Transplantation of neonatal dog peripheral mononuclear cells into the infarcted regions can increase the capillary density and decrease infarcted size, preserving left ventricular function.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2004年第2期179-181,共3页
Academic Journal of Second Military Medical University
