摘要
目的 研究机体在正常和带瘤情况下免疫功能状态 ,动态了解手术切除肿瘤后 T淋巴细胞亚群数量和功能两方面的变化 ,并探讨肿瘤患者术后感染与其机体细胞免疫水平改变的相互关系。方法 分别用 3H- Td R渗入法和单个核细胞直接免疫荧光法检测 38例胃癌患者 Ts功能和 T淋巴细胞亚群水平 ,并对手术切除后不同时间点进行了对比。结果 胃癌患者无论手术前、后 Ts功能明显高于正常人 ( P<0 .0 1 ) ,而胃癌患者本身在手术切除肿瘤前、后 Ts功能无显著变化 ( P>0 .0 5 ) ;Ts功能与 CD3、CD8呈正相关 ( P<0 .0 5 ) ,与 CD4、CD4/CD8呈直线负相关 ( P<0 .0 0 1 ) ;胃癌患者术前 CD3、CD4明显低于正常人( P<0 .0 1 ) ,CD8略有增加 ,但不明显 ( P<0 .0 5 ) ,而 CD4/CD8明显降低 ( P<0 .0 0 1 ) ;肿瘤切除后 CD3稍有增加 ( P>0 .0 5 ) ,CD4呈下降趋势 ( P<0 .0 5 ) ,术后 6～ 8d为最低值 ,CD8则轻度增高 ,CD4/CD8先降后升 ,这些变化均在术后 9～ 1 2 d恢复到术前水平 ,并逐步接近正常水平。TH变化与术后感染的相关性研究表明 :术后 2～ 3d,CD4下降病人的感染率为 43.5 % ,TH增高病人的感染率为 1 3.3%。结论 胃癌患者的细胞免疫功能明显低下 ,并随病情发展逐步减低。手术切除肿瘤组织有利于机体免疫功能的恢复 ,但?
Objective To investigate the quantity and function of T lymphocytes subsets in preoperation and postoperation of patients with gastric cancer and subsequently,to consider the mechanism of association between postoperative infection and variation of cellular immunity.Methods The Ts function and T lymphocytes subsets level were detected respectively by 3H-TdR and direct immunofluorescence technique in 38 patients with gastric cancer.And the results of different time points were compared.Results The Ts function of patients with gastric cancer,whether preoperation or postoperation was remarkably higher than that of the normal(P<0.01),however,no significant difference was seen between the preoperation and postoperation(P>0.05),the Ts function bore a parallel relationship to the level of CD3,CD8(P<0.05) and a straight negative relationship with the level of CD4, CD4/CD8(P<0.001).There was a significant decrease in CD3 and CD4 level in preoperation of patients with gastric cancer in comparison with that of the normal (P<0.01).The level of CD8 was slightly above the normal value,but unremarkable (P>0.05).The CD4/CD8 was significantly depressed(P<0.001).After surgical removal of tumor,the level of CD3 increased slightly (P>0.05),and the downward trends appeared in the CD4 level (P<0.05) and the smallest level occurred in 6～8 days postoperatively.The CD8 level increased slightly,however,the CD4/CD8 was depressed at beginning of postoperation,followingly increased.All of these variation were restored to the level of preoperation and to normal gradually.The study on association between postoperative infection and variation of TH showed that the rate of infection was 43.5% in the patients with CD4 depression and 13.3% in CD4 increassion in 2～3 days postoperatively.Conclusion The cellular immunity is significantly low in patients with gastric cancer and diminish gradually as the illness progresses.The surgical removal of tumor is favourable for restoration of the immune function,but the surgical lesion can result in so
出处
《现代检验医学杂志》
CAS
2004年第1期22-24,共3页
Journal of Modern Laboratory Medicine
关键词
胃癌
T淋巴细胞亚群
Ts功能
术后感染
gastric cancer
T lymphocytes subsets
Ts function
postoperation infection
