摘要
目的:研究葡多酚(GPC)对DNA氧化损伤防护作用。方法: 将大鼠分为正常对照组、高脂膳食、高脂+乙醇、高脂+ GPC(50 mg/kg bw)、高脂+乙醇+ GPC(50 和100 mg/kg bw)等共6组,饲养6 w,处死动物,制作脾细胞悬液,分不给和给予Fenton氧化两种处理,测定脾细胞DNA损伤程度及血清MDA水平和SOD活力等指标。结果: 1.不给予Fenton氧化处理的高脂+乙醇组和高脂+乙醇+GPC(100 mg/kg bw)组的 DNA损伤程度分别为76.15%和15.27%,给予氧化处理的为83.02%和19.83%,差异均有显著意义。2.高脂+乙醇组和高脂+乙醇+GPC(100 mg/kg bw)组的MDA分别为8.18±0.98 mmol/L和5.63±1.16 mmol/L,SOD为597.04±88.88 NU/ml和729.71±88.88 NU/ml,差异均有显著意义。结论: GPC对高脂和乙醇引发的DNA氧化损伤有防护作用。
Objective: To study the protective effect of grape procyanidins(GPC) on DNA oxidative damage. Methods:Adult Wistar rats were divided into six groups including normal control group(N), high fat (F) group, F+alcohol (A) group, F+GPC group, FA+GPC( 100 mg/kg and 50 mg/kg) group. Kill all rats after 6 w, and examine DNA damage of spleen cell that was treated or untreated with Fenton system and serum MDA level and SOD activity. Results: 1. DNA damage degree of FA group and FA+GPC (100mg/kg) were 83.02%,19.83% when treated with Fenton system and 76.15%, 15.27% when untreated respectively. 2. MDA level in FA group and FA+GPC (100 mg/kg) group were 8.18±0.98 mmol/L and 5.63±1.16 mmol/L,SOD activity were 597.04±88.88 NU/ml and 729.71±88.88 NU/ml respectively. Conclusion:GPC had favourable protective effect on oxidation damage induced by high fat or alcohol.
出处
《营养学报》
CAS
CSCD
北大核心
2004年第1期54-56,共3页
Acta Nutrimenta Sinica
基金
国家自然科学基金(NO.39870678)
