摘要
目的 :探讨端粒酶基因在颈部淋巴结病变中的表达及临床意义。方法 :采用端粒酶基因原位杂交技术检测93例颈部淋巴结病变细针吸取的细胞。结果 :93例颈部淋巴结病变中 ,阳性总表达率为 73.12 % (6 8/ 93)。淋巴结转移癌占 10 0 % (33/ 33) ,非何杰金淋巴瘤占 10 0 % (3/ 3) ,增生性淋巴结炎占 6 2 .5 % (2 5 / 4 0 ) ,慢性淋巴结炎占 5 0 %(6 / 12 ) ,亚急性淋巴结炎占 2 0 % (1/ 5 )。结论 :在细针吸取细胞学中应用国产端粒酶基因原位杂交检测技术简单可行 ,对颈部淋巴结病变的诊断及鉴别诊断有一定的临床价值。
Objective:To establish the method for detection of telomerase genes by in situs hybridization and to investigate expression and prognostic significance of telomerase genes activity in lymphonodus lump by fine-needle aspiration cytology.Methods:Ninety-three cases of lymphonodus lump were examined for expressions of telomerase genes by in situs hybridization and by fine-needle aspiration.Results:The total positive expression rate was 73%(68/93),metastatic carcinoma of lymphonode was 33/33 cases(positive intension+++---++++),non-Hodgkin lymphoma was 3/3 cases(positive intension+++ ),hyperplastic lymphadenitis was 25/40 cases(±---+++),chronic lymphadenitis was 6/12 cases(positive intension±---++),subacute lymphadenitis was 1/5 cases(positive intension±---++).The malignant lump had obviously significant difference from benign lump in positive rates and intension(P<0.01).Conclusion:The detection of telomerase genes by in situs hybridization with fine-needle aspiration cytology shows an great clinical value in disclosing malignant tumor and benign tumor.It is also simple in operation.
出处
《实用临床医学（江西）》
CAS
2004年第1期24-26,共3页
Practical Clinical Medicine
关键词
淋巴结
端粒酶
细胞学
原位杂交
lymphonodus
telomerase
cytoloy,situshybridization
