摘要
目的 探讨某些细胞因子在慢性乙型肝炎 (CHB)患者肝纤维化中的作用 ,为血清学无创伤早期诊断CHB患者的肝纤维化提供新指标。方法 分别用 EL ISA法检测 1 2 9例慢性乙型肝炎患者 (其中轻度 4 9例 ,中度 5 7例 ,重度 2 3例 )血清 IL- 6、IL- 1 0、IL- 1 8水平 ,RIA法检测 HA、PC 浓度 ,并分析它们之间的相关关系。结果 1 2 9例慢性乙型肝炎患者 (轻度、中度、重度 )血清 IL- 6 (pg/ml)、IL- 1 0 (pg/ml)、IL- 1 8(pg/ml)、HA(ng/m l)、PC (μg/ml)浓度分别是 1 3.6± 4 .75、1 9.8± 8.76、4 2 .5± 1 3.5 (分别与正常组比较 P<0 .0 1 ,组间比较 P<0 .0 5 ) ;4 4 .2± 1 6 .4、35 .6± 1 7.8、2 8.5± 1 0 .2 (分别与正常组比较 P<0 .0 5 ,组间比较 P<0 .0 5 ) ;89.4± 2 4 .4、1 74 .5± 77.3、2 6 5 .8± 1 0 4 .3(分别与正常组比较 P<0 .0 1 ,组间比较 P<0 .0 5 ) ;1 80 .4± 85 .2、2 36 .2± 1 36 .6、32 5 .4± 1 5 6 .4 ,1 1 3.1± 75 .3、1 5 9.3±1 0 8.9、2 35 .1± 1 2 5 .7。 32例正常对照上述血清指标则分别为 5 .4± 3.7、2 1 .4± 8.1、4 6 .8± 1 2 .5、5 7.4± 2 8.6、90 .2±36 .1。相关分析表明慢性乙型肝炎患者血清 HA、PC 水平与 IL- 6、IL- 1 0、IL- 1 8浓度的相关系数
Objective To study the effect of some cytokins on hepatic fibrosis in patients with chronic hepatitis B(CHB) and to provide a new indicator for early and noninvasive serologic diagnosis of hepatic fibrosis.Methods The concentration of serum IL 6,IL 10,IL 18 were detected by ELISA and hyaluronic acid(HA),procollagen type Ⅲ(PCⅢ) by RIA,respectively in 129 cases of CHB(mild 49 cases,moderafe 57 cases,severe 23 cases).Results The concentrations of serum IL 6(pg/ml) of 129 cases(mild,moderate,severe)were 13.6±4.75,19.8±8.76,42.5± 13.5 ,respectively(compared with controls, P <0.01;compared with other cases, P < 0.05 );IL 10(pg/ml) were 44.2±16.4,35.6±17.8,28.5±10.2(compared with controls, P < 0.05 ;compared with other cases, P < 0.05 );IL 18(pg/ml) were 89.4±24.4,174.5±77.3,265.8±104.3(compared with controls, P <0.01;compared with other cases, P <0.05);HA(ng/ml) were 180.4±85.2,236.2±136.6,325.4±156.4;and PCⅢ(μg/ml) were 113.1±75.3,159.3±108.9,235.1±125.7.In 32 cases of controls the concentrations of serum IL 6?IL 10?IL 18,HA and PCⅢ were 5.4±3.7,21.4±8.1,46.8±12.5,57.4±28.6,90.2±36.1 respectively.Correlation coeficient( r ) of concentration of HA,PCⅢ with IL 6,IL 10,IL 18 were 0.636( P <0.01)?-0.303( P <0.05),0.679( P <0.01); 0.273 ( P <0.05),-0.132( P >0.05), 0.282 ( P < 0.05 )respectively.Conclusion Cytokines such as IL 6,IL 10,IL 18 may have important effect on hepatic fibrosis in patients with chronic hepatitis B by regulating the activity of TH1/TH2 cells,and have clinical significance in diagnosis of hepatic fibrosis.Inhibition of the activity of IL 6,IL 18 and enhencement of IL 10 may be an efficient approach of preventing hepatic fibrosis.
出处
《淮海医药》
2004年第2期87-89,共3页
Journal of Huaihai Medicine
