摘要
目的 探讨西咪替丁对胃癌细胞MKN45生长的抑制和凋亡作用。方法 采用MTT比色法测定不同浓度西咪替丁作用下胃癌细胞MKN45培养液的OD值 ,并用流式细胞仪测定西咪替丁对MKN45的细胞周期和细胞凋亡的影响。结果 西咪替丁作用 72小时 ,抑制胃癌细胞MKN45的生长并呈剂量依赖趋势。胃癌细胞MKN45细胞周期发生改变 ,G1期百分比减少 ,S期百分比增加 ,并出现明显的细胞凋亡峰。结论 西咪替丁可能通过诱导胃癌细胞MKN45凋亡从而抑制其细胞增殖。
Objective To study cimetidine's effect on proliferation and apoptosis of gastric cancer cell MKN45.Methods The proliferation of gastric cancer cell line MKN45 induced by cimetidine was determined by MTT. The cell cycle and apoptosis ration of MKN45 cell were determined by flow cytometry in the presence of cimetidine.Results Cimetidine inhibited dose dependingly the proliferation of gastric cancer cell MKN45. Significant alteration with decreasing percentage of GI phase and increasing percentage of S phase of gastric cancer cell MKN45 could be detected after incubating 72 hous with cimetidine.Conclusions Cimetidine could inhibit the proliferation of gastric cancer cell MKN45 by inducing its apoptosis.
出处
《胃肠病学和肝病学杂志》
CAS
2004年第1期63-66,共4页
Chinese Journal of Gastroenterology and Hepatology
