摘要
目的 :观察中药复方四毒清对脂多糖 (LPS)攻击小鼠生存率和器官功能的影响 ,寻找有效中药防治LPS性多器官功能衰竭。方法 :将中药四毒清按常规方法煎制成 1kg/L药液 ,以小鼠为实验对象 ,灌胃给药 ,3d后腹腔注射LPS(6 0mg/kg) ,观察小鼠生存率。另分批进行同样实验 ,观察小鼠血清丙氨酸转氨酶 (ALT)活性、血清肌酐(Cr)和尿素氮 (BUN)含量及肺、肝、肾、肠的病理变化。结果 :LPS注射后 96h ,LPS组小鼠的死亡率为 73% (n =34) ,而四毒清防治组小鼠的死亡率为 35 % (n =31) ,两组死亡率有明显差别 (P <0 0 5 )。同时 ,LPS组小鼠的血清ALT活性、Cr和BUN含量明显高于对照组 ,而四毒清防治组小鼠血清ALT活性、Cr和BUN含量明显低于LPS组。病理学检查发现四毒清防治组小鼠肺、肾及小肠病变明显轻于LPS组。结论 :中药四毒清能防治LPS性多器官功能障碍 ,提高LPS攻击小鼠的生存率。
AIM: To investigate the effect of siduqing decoction,a Chinese medicine,on survival rate and multiple organ dysfunction in mice challenged with LPS. METHODS: Mice were administered intragastrically with Siduqing decoction or distilled water (0.2 ml/10 g) twice a day for 3 days,two hours after Chinese herbal medicine treatment on day 3,LPS or normal saline was injected intraperitoneally,and survival rates in each group were recorded at 12-h intervals. In another experiment,mice were sacrificed at 12 h after LPS,lung,liver,kidney and small intestine were collected and processed for the H & E staining. In addition,Blood was collected at 10 h after LPS injection for determining alanine aminotransferase (ALT) activity,blood urea nitrogen (BUN) and creatinine (Cr) contents. RESULTS: At 96 h after LPS injection,the survival rate (27%, n =34) was lower in LPS group than Siduqing treatment group (65%, n=31,P<0.05 ). ALT activity,BUN and Cr contents in serum were higher in LPS group than control group,Siduqing treatment significantly attenuated a increase in ALT activity,BUN and Cr content in serum induced by LPS. Histological examination showed inflammatory injury in the lung and intestine,hemorrhage in the lung and kidney,degeneration,necrosis in the liver and kidney,while Siduqing treatment attenuated pathological changes induced by LPS. CONCLUSION: These data indicate that Siduqing has a protective effect against LPS-induced multiple organ injury and increases survival rate in mice challenged with LPS.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第2期219-223,共5页
Chinese Journal of Pathophysiology
基金
20 0 3年暨南大学团队项目
广东省中医药管理局资助项目 (10 30 4 4 )
广东省自然科学基金资助项目(31870 )
关键词
脂多糖类
多器官功能衰竭
中草药
活存率
Lipopolysaccharides
Multiple organ failure
Drugs,Chinese herbal
Survival rate
