摘要
目的:探讨谷氨酸及其N-甲基(酰)-D-门冬氨酸受体(NMDAR1)在大鼠前额叶执行控制功能中的作用机制。方法:1.0～1.5月龄的大鼠,进行执行控制训练,选出执行控制能力好组及执行控制能力差组大鼠各8只,用氨基酸分析技术、免疫组化技术和原位杂交技术研究执行控制能力不同的大鼠前额叶谷氨酸(Glu)含量、Glu免疫反应阳性神经元(Glu-IR)数量和N-甲基-D-天冬氨酸受体1mRNA(NMDAR1mRNA)表达。结果:与执行控制能力好组大鼠相比,执行控制能力差组大鼠NM-DAR1mRNA表达较高犤执行控制能力好组大鼠为(117±33)个/μm2,控制能力差组为(165±28)个/μm2,t=1.93,P<0.05犦;而两组间前额叶Glu含量犤(11.9±0.6)μmol/g比(12.4±0.9)μmol/g犦,Glu-IR阳性神经元数量犤(197±94)个/μm2比(212±87)个/μm2;t=1.21,P>0.05犦差异无显著性意义(t=1.21,P均>0.05)。结论:Glu及NMDAR1在前额叶执行控制中起重要作用,机制可能比较复杂,NMDAR1mRNA表达较高可能系代偿的结果。
AIM:To study the effect and mechanism of glutamic acid(Glu) and NMDAR1 in prefrontal cortex executive control of rats. METHODS:Sixteen rats(1.0-1.5 months) were trained by prefrontal cortex executive control and divided into two groups(rats with poor executive control, n=8 and rats with good executive control,n=8 ).The technique of amino acid analysis, immunohistochemistry and in situ hybridization were used to measure Glu content, the number of Glu IR(immunologic reaction) positive neurons and NMDAR1mRNA expression. RESULTS:Compared with rats with good executive control,rats with poor executive control had more NMDAR1mRNA expression[(117±33)/μm2 vs (165±28)/μm2;t=1.93,P< 0.05].There were no significant differences in Glu content[(11.9±0.6)μmol/g and (12.4±0.9) μmol/g]and the number of Glu IR positive neurons[(197±94)/μm2 and(212±87)/μm2;t=1.21,P >0.05]. CONCLUSION:Glu and NMDAR1 play an important role in prefrontal cortex executive control. The mechanism is complicated. The higher expression of NMDAR1mRNA can be compensatory effect.
出处
《中国临床康复》
CSCD
2003年第31期4202-4203,共2页
Chinese Journal of Clinical Rehabilitation
