摘要
在L 酒石酸衍生的键合网状聚合物HPLC手性固定相上正相拆分了 7种氨基酸衍生物 ,考察了流动相中极性醇添加剂的种类、含量和柱温等对手性拆分效果的影响 ,结合计算得的热力学函数对手性识别机理进行了探讨。当流动相为正己烷∶异丙醇 (95∶5 ) ,流速 1mL/min ,柱温 2 5℃时 ,7种底物对映分离效果好 ,出峰时间均在
Seven amino acid derivatives were resolved on a high performance liquid chromatograpic chiral stationary phase consisting of immobilized network polymer derived from L-tartaric acid in the normal-phase mode. The effects of the column temperature, the type and concentration of the polar alcohol modifier on the chiral resolution were studied. Associated with the calculated thermodynamic parameters, the mechanism of chiral recognition was discussed. All these amino acid derivatives can be baseline separated using hexane/2-propanol(95∶5) as eluent, at a flow rate of 1 mL/min and at 25℃, with reasonable retention times(<12 min).
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2004年第2期213-216,共4页
Chinese Journal of Analytical Chemistry
基金
福建省高校重点科技项目基金 (No .JA992 10 )
福建省科技厅三项经费 (No .K0 2 0 92 )资助
