摘要
采用气相色谱内标法测定鱼油保健品中的EPA和DHA含量,用酯交换法对样品进行处理,对程序升温条件进行优化,通过考察线性关系、精密度、重复性和稳定性、加标回收等对已建立的方法进行验证,并对市售10个不同厂家生产的鱼油保健品进行EPA和DHA含量评价。结果表明:EPA和DHA分别在5. 57~278. 50μg/m L和6. 97~348. 50μg/m L质量浓度范围内与峰面积呈良好的线性关系(相关系数分别为0. 999 6和0. 999 1),精密度(相对标准偏差(RSD)分别为0. 43%和1. 05%)、重复性(RSD分别为1. 04%和1. 62%)、稳定性(RSD分别为1. 48%和1. 27%)、准确性(平均回收率分别为100. 47%和101. 43%)良好。10个鱼油保健品样品质量参差不齐,需要完善的质量规范。所建立的检测方法准确性好、精密度高,可用于鱼油保健品中EPA和DHA含量的检测与质量控制。
The contents of DHA and EPA in fish oil health products were determined by gas chromatography internal standard method.The samples were treated by transesterification and the chromatography condition was optimized.The method was verified by linear relationship,precision,repeatability,stability and recovery of standard.The contents of DHA and EPA in fish oil health products from ten different manufacturers were evaluated.The results showed that mass concentrations and peak areas of EPA and DHA had good linear relationships in ranges of 5.57-278.50μg/m L and 6.97-348.50μg/m L respectively(R2=0.999 6,0.999 1).The precision(RSD=0.43%,1.05%),repeatability(RSD=1.04%,1.62%),stability(RSD=1.48%,1.27%),and accuracy(recovery rate 100.47%,101.43%)were good.The qualities of ten samples were uneven,and the quality specification should be improved.The method showed good accuracy and high precision,which could be used for the determination of EPA and DHA contents in fish oil health products.
作者
朱丽君
王鲁霞
武玲
赵宝义
范美霞
姜祎頔
ZHU Lijun;WANG Luxia;WU Ling;ZHAO Baoyi;FAN Meixia;JIANG Yidi(Heze Administrative Examination and Approval Service Bureau,Heze 274000,Shandong,China;Heze Institute for Food and Drug Control,Heze 274000,Shandong,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2019年第8期130-133,共4页
China Oils and Fats
关键词
气相色谱
内标法
鱼油
EPA
DHA
gas chromatography
internal standard method
fish oil
EPA
DHA
