摘要
二十二碳六烯酸(DHA)属于ω-3不饱和脂肪酸,是保持人体健康的重要营养成分之一,具有重要的医药应用和营养价值。与植物和动物来源相比,裂殖壶菌合成DHA的工业应用前景广阔。研究表明,过表达聚酮合成酶(Polyketide synthase,PKS)和脂肪酸合成酶(Fatty acid synthase,FAS)途径中的关键作用酶基因与改变培养条件(如碳源、氮源、温度、无机盐、微量元素、前体物质及生物促进剂等),均可显著提高DHA产量。此外,不同限制条件下的裂殖壶菌基因组及转录组的测序分析,揭示了DHA合成过程种不饱和双键形成、信号传导、脂质转运及代谢的分子机制。
Docosahexaenoic acid(DHA),which belongs to omega-3 unsaturated fatty acid,is one of the important nutrients for maintaining human health with potential medical applications.Compared with plant and animal sources,DHA synthesized by Schizomycetes has broaden the industrial application prospects.Studies showed that there were some promising ways to improve the DHA production,including over expressing the key enzymes of polyketide synthase(PKS)and fatty acid synthase(FAS)pathway,as well as optimizing the cultivation conditions(such as carbon sources,nitrogen sources,temperature,inorganic salts,trace elements,precursor substances and biological promoters).In addition,sequencing and analysis of the genome and transcriptome of Schizomycetes under different conditions further revealed the mechanism of DHA synthesis,corresponding to the unsaturated double bond formation,signal introduction,lipid transportation and metabolism.
作者
杨浩
金小琬
唐嘉淇
黄健庭
陈天晓
杨雪薇
黎双飞
YANG Hao;JIN Xiaowan;TANG Jiaqi;HUANG Jianting;CHEN Tianxiao;YANG Xuewei;LI Shuangfei(Guangdong Key Laboratory of Plant Epigenetics,College of Life Science and Oceanography,Shenzhen University,Shenzhen 518055,Guangdong,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2019年第8期109-115,共7页
China Oils and Fats
基金
深圳市孔雀技术创新项目(201890929)
深圳市战略新兴和未来产业发展资金(201708021308)
深圳市基础研究自由探索项目(JCYJ20170302144706218)
深圳大学新引进高端人才科研启动项目
深圳大学新引进教师科研启动项目(2016100)
