摘要
目的观察芍药软肝合剂对H22肝癌小鼠免疫功能的影响,并初步探讨芍药软肝合剂对及VEGF、PCNA表达的影响。方法采用细胞接种的方法建立小鼠H22肝癌动物模型,随机分为正常组、模型组、环磷酰胺组、中药低、中、高剂量组,观察各组动物的整体情况,行免疫组化染色实验对肿瘤组织中VEGF、PCNA表达进行检测。结果 1)成功建立小鼠H22肝癌动物模型;2)芍药软肝合剂提高荷瘤小鼠机体免疫功能;3)芍药软肝合剂能降低H22肝癌小鼠肿瘤组织中VEGF、PCNA的表达水平。结论芍药软肝合剂对H22荷瘤小鼠的胸腺、脾脏有保护作用,能提高荷瘤小鼠机体免疫功能。其能抑制荷瘤小鼠中VEGF、PCNA蛋白的表达,从而抑制肿瘤生长。
Objective: To observe the immune effec of Shaoyao Ruangan Recipe on hepatocarcinoma H22 tumor-bearing mice and to preliminary discuss the express influence of Shaoyao Ruangan Recipe to Proteins such as VEGF、PCNA and In order to clarify the Mechanism of Hepatocellular Carcinoma with Shaoyao Ruangan Recipe. Methods: Animal models of H22 hepatic cancer with mice were built according to inoculate tumor cell.Mice were randomly divided into six groups such as the normal group,the model group,CTX group,low-dose,middle-dose and high-dose group of Shaoyao Ruangan Recipe.Observe the mices’ overall condition. The tumor growth inhibition rate spleen index was determined separately. The tumor tissue were carried out by the immune dyeing experiment to determine the protein express of VEGF、PCNA in the tumor tissue. Results: 1) The animal model of Hepatocarcinoma H22 Tumor-bearing Mice was set up successfully. 2)Shaoyao Ruangan Recipe could improve the immunity of tumor-bearing mice. 3)The middle-dose of Shaoyao Ruangan Recipe could reduce the expression of VEGF and PCNA. Conclusions: Shaoyao Ruangan Recipe can protect the spleen and thymus gland of tumor-bearing mice,also has the ability to increase the immunity of tumor-bearing micec. Its anti-tumor mechanism may relate to the control of VEGF、PCNA express in tumor-bearing mice.
出处
《中国中医急症》
2015年第4期590-592,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
浙江省中医药科技计划项目(2010ZA007)
