摘要
目的:观察加味芍药甘草汤对多囊卵巢综合征(PCOS)大鼠的干预作用及探讨其可能的作用机制。方法:SD雌性大鼠随机分为正常组、模型组、达英-35(0.203 5 g/kg)组、加味芍药甘草汤(50 g/kg)组,每组8只。除正常组外,其余3组灌胃来曲唑(0.001 g/kg)连续21 d制备PCOS模型,造模成功后达英-35组和加味芍药甘草汤组予以药物灌胃14 d,正常组和模型组予以相应体积生理盐水。实验过程中测量大鼠体质量并观察动情周期变化;腹主动脉取血和采集双侧卵巢并称重,酶联免疫吸附(ELISA)法测定血清性激素(睾酮、雌二醇、促黄体生成素、卵泡刺激素、孕酮)和炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、IL-6、IL-18的含量;苏木素-伊红(HE)染色观察卵巢组织病理学改变;实时荧光定量PCR法测定卵巢组织核转录因子-κB(NF-κB)、NF-κB p65、κB抑制因子-α(IκB-α)、TNF-α、IL-1β、IL-6、IL-18 mRNA表达水平;蛋白免疫印迹法检测大鼠卵巢组织phospho(P)-NF-κB p65、IκB-α蛋白表达水平。结果:与正常组比较,模型组大鼠体质量和卵巢重量明显增加,动情周期紊乱,血清睾酮和促黄体生成素含量明显增加,而雌二醇和卵泡刺激素显著下降,血清TNF-α、IL-1β、IL-6、IL-18含量显著增加,卵巢组织NF-κB、NF-κB p65、TNF-α、IL-1β、IL-6、IL-18 mRNA及P-NF-κB p65蛋白表达水平明显升高,而IκB-αmRNA及蛋白表达水平显著下降(P<0.05);与模型组比较,加味芍药甘草汤组体质量和卵巢重量明显减轻,动情周期和血清性激素水平重新恢复正常,血清TNF-α、IL-1β、IL-6、IL-18含量显著减少,卵巢组织NF-κB、NF-κB p65、TNF-α、IL-1β、IL-6、IL-18 mRNA及P-NF-κB p65蛋白表达水平明显降低,而IκB-αmRNA及蛋白表达水平显著升高(P<0.05),卵巢组织结构明显改善。结论:加味芍药甘草汤能够明显改善PCOS大鼠多囊卵巢形态和功能并调节卵巢激素微环境,其作�
Objective:To investigate the intervention effect and potential mechanism of Modified Shaoyao Gancao Decoction on polycystic ovary syndrome(PCOS)in rats.Methods:SD female rats were randomly dividedinto normal group,model group,Dinae-35(0.203 5 g/kg)group and Modified Shaoyao Gancao Decoction(50 g/kg)group,with 8 rats in each group.Except for the normal group,all of the other groups were treated with letrozole(0.001 g/kg)for 21 days to prepare PCOS model.After successful modeling,Dinae-35 group and Modified Shaoyao Gancao Decoction group were orally given the drug for consecutively 14 days,and normal group and model group were given the equal volume of normal saline.During the experiment,the body weight of the rats was measured and the estrous cycle was observed.The contents of serum sex hormones(testosterone,17β-estradiol,luteinizing hormone,follicle stimulating hormone,progesterone),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),IL-6 and IL-18 were determined by enzyme-linked immunosorbent assay(ELISA).The pathological changes of ovarian tissues were observed by hematoxylin-eosin(HE)staining.The levels of nuclear factor-κB(NF-κB),NF-κB p65,inhibitor ofκB(IκB-α),TNF-α,IL-1β,IL-6 and IL-18 mRNA expressions in ovarian tissues were determined by real-time quantitative PCR method.The levels of phospho(P)-NF-κB p65 and IκB-αprotein expressions were detected by Western blot.Results:Compared with the normal group,the model group significantly increased body weight and ovarian weight with irregular estrous cycle.The contents of serum testosterone and luteinizing hormone were significantly increased,while the contents of serum 17β-estradiol and follicle stimulating hormone were significantly decreased.The contents of serum TNF-α,IL-1β,IL-6 and IL-18 were increased significantly.The levels of NF-κB,NF-κB p65,TNF-α,IL-1β,IL-6,IL-18 mRNA and P-NF-κB p65 protein expressions in ovarian tissues were significantly increased,whereas the levels of IκB-αmRNA and protein expression were significantly d
作者
常壮鹏
邵云云
刘俊瑾
程瑶
侯锐钢
CHANG Zhuangpeng;SHAO Yunyun;LIU Junjin;CHENG Yao;HOU Ruigang(School of Pharmacy,Shanxi Medical University,Taiyuan 030000,Shanxi,China;The Second Hospital of Shanxi Medical University,Taiyuan 030000,Shanxi,China)
出处
《中华中医药学刊》
CAS
北大核心
2019年第9期2295-2300,2322,共7页
Chinese Archives of Traditional Chinese Medicine
基金
山西省科技攻关项目(2014ZD0102,2016ZD0303)
关键词
加味芍药甘草汤
多囊卵巢综合征
核转录因子-ΚB
炎症因子
Modified Shaoyao Gancao Decoction
polycystic ovary syndrome
nuclear factor-κB
inflammatory factor
