摘要
MpMYB96是拟南芥(Arabidopsis thaliana) AtMYB96的一个同源基因。为了探究MpMYB96在苹果(Malus pumila)中的功能,本文以‘皇家嘎拉’苹果苗的cDNA为模板进行PCR扩增,获得一个约1 000 bp的目的条带,通过测序发现该基因长为1 029 bp,序列与MpMYB96 (MDP0000144744, https://www.rosaceae.org/)的序列一致,该蛋白序列含有两个SANT保守结构域。进化树分析了15个物种MYB96蛋白的亲缘关系,表明MpMYB96在不同物种中的功能是保守的。此外,结果发现苹果MpMYB96与白梨(Pyrus bretschneideri) PbMYB96的亲缘关系最近,说明MpMYB96和PbMYB96可能具有相同的功能。实时荧光定量PCR发现MpMYB96在花中表达量最高,茎中表达量次之,根和叶中表达量均较低,暗示MYB96可能在花中扮演更重要的角色。MpMYB96的表达受脱落酸(ABA)、聚乙二醇(PEG)、水杨酸(SA)处理的诱导,表明MpMYB96的功能可能涉及到了ABA和SA以及PEG模拟的干旱过程。将MpMYB96转化苹果愈伤组织,检测转基因愈伤的表型,发现MpMYB96过表达能够增加植物对ABA和PEG的敏感性,并且能够对轮纹病产生一定的抵抗能力。
MpMYB96 is a homologous gene of Arabidopsis thaliana AtMYB96.In order to explore the function of MpMYB96 in apple(Malus pumila),the cDNA of the apple seedling was used as the template for PCR amplification in this paper,and a target band of about 1 000 bp was obtained.Through sequencing,it was found that the gene was 1 029 bp in length,and is identical to the sequence of MpMYB96(MDP0000144744,https://www.rosaceae.org/).The protein sequence contains two SANT conserved domains.The phylogenetic tree analysis of MYB96 protein in 15 species shows that the function of MYB96 in different species is conservative.In addition,it was found that apple MpMYB96 is most closely related to PbMYB96(Pyrus bretschneideri),suggesting that MpMYB96 and PbMYB96 may have the same function.Real-time fluorescence quantitative PCR showed that the expression of MpMYB96 was highest in flowers,followed by that in stems,and lower in roots and leaves,suggesting that MYB96 may play a more important role in flowers.The expression of MpMYB96 was induced by abscisic acid(ABA),polyethylene glycol(PEG)and salicylic acid(SA)treatments,suggesting that the function of MpMYB96 may involve ABA and SA,as well as the simulated drought process of PEG.MpMYB96 was transformed into apple callus,and the phenotype of the transgenic callus was detected.It was found that the overexpression of MpMYB96 could increase the plant’s sensitivity to ABA and PEG,as well as develop a certain resistance to Botryosphaeria dothidea.
作者
赵先炎
齐晨辉
姜翰
钟明爽
李媛媛
郝玉金
ZHAO Xian-Yan;QI Chen-Hui;JIANG Han;ZHONG Ming-Shuang;LI Yuan-Yuan;HAO Yu-Jin(College of Horticulture,Northwest A&F University,State Key Laboratory of Crop Stress Biology for Arid Areas,Shaanxi Key Laboratory of Apple,Yangling,Shaanxi 712100,China;College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency,Ministry of Agriculture Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region,Taian,Shandong 271018,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2019年第6期783-792,共10页
Plant Physiology Journal
基金
国家自然科学基金(31772275)
山东省自然科学基金优青项目(ZR2018JL014)~~
