摘要
目的初步探究人类无远端同源框基因家族5 (distal-less homeobox,DLX 5)是否与胶质瘤病人预后相关及可能机制。方法采用来自中国脑胶质瘤基因图谱数据库(CGGA)的83个原发胶质母细胞瘤(primary glioblastoma multiform,pGBM)病人进行全基因组表达芯片检测。结果在m RNA表达谱中鉴定出两组样本(称为G1组和G2组)。G1组富集基因主要与神经细胞分化和发育有关,G2组富集基因主要发挥免疫功能。在所有相关富集基因中,DLX5与病人预后显著相关(P <0.001,OR=1.255)。在83例pGBM病人中,DLX5高表达病人中位生存期47.8周,DLX5低表达病人中位生存期72.9周,DLX5高表达病人生存率较低(P <0.01)。在长生存期pGBM病人中,DLX5甲基化水平较高,且mi RNA表达水平与DLX5表达量有明显相关性。结论 DLX5高表达可作为pGBM病人不良预后标志物,为pGBM病人药物研发提供潜在靶点。
Objective To explore whether the human distal-less homeobox 5(DLX5)gene is associated with prognosis and potential mechanisms in patients with glioma.Methods This research has performed whole genome expressed gene microarray in 83 primary glioblastoma multiforme(pGBM)patients from the Chinese Glioma Genome Atlas(CGGA).Results Two major groups of samples were identified(referred to as G1 group and G2 group).The enriched genes were associated with differentiation and development of neuronal cells in G1 group.Immunity associated genes were enriched in G2 group.One of the most significant prognostic genes was DLX5(P<0.001,OR=1.255).In 83 patients with pGBM,the median survival time was 47.8 weeks in high DLX5 expression,and 72.9 weeks in low DLX5 expression.Overexpression of DLX5 indicated poor survival(P<0.01).DLX5 methylation was higher in long-term survival pGBM patients,and mi RNA expression levels were significantlycorrelated with DLX5 expression.Conclusion High expression of DLX5 can be used as a poor prognostic marker for pGBM patients,providing a potential target for drug development in pGBM patients.
作者
保肇实
王强威
王永志
Bao Zhaoshi;Wang Qiangwei;Wang Yongzhi(Department of Neurosurgery,Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,China;Department of Molecular Pathology,Beijing Neurosurgical Institute,Beijing 100070,China)
出处
《中国微侵袭神经外科杂志》
CAS
2019年第8期364-369,共6页
Chinese Journal of Minimally Invasive Neurosurgery
基金
国家自然科学基金(编号:81502606、81773208)
北京市科技新星计划(编号:Z171100001117022)
北京市青年拔尖计划课题(编号:2017000021223ZK32)
北京天坛医院青年科学家计划(编号:YSP201701)
