摘要
菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)和玉米褪绿斑驳病毒(Maize chlorotic mottle virus,MCMV)是我国进境植物检疫性有害生物。2018年6月秦皇岛海关通过反转录PCR(RT-PCR)和双抗体夹心酶联免疫吸附(DAS-ELISA)方法,从一批美国进口大豆以及杂质中检出BPMV和MCMV;同时利用实时荧光PCR(Real time RT-PCR)和序列比对分析方法进行验证。这是河北口岸首次在进境大豆中截获BPMV和MCMV。
Bean pod mottle virus(BPMV)and Maize chlorotic mottle virus(MCMV)are two important quarantine pests of China.BPMV and MCMV were detected in soybeans and impurities imported from USA using reverse transcription PCR(RT-PCR)and double-antibody sandwich enzyme-linked immunosorbent assay(DAS-ELISA)methods respectively by Qinhuangdao Customs in June 2018,which was further confirmed by Real time RT-PCR and sequence alignment analysis.This was the first interception of BPMV and MCMV from imported soybean in Hebei Province.
作者
柳吉芹
王立杉
吴曦
钱云开
高飞
王海洋
Liu Jiqin;Wang Lishan;Wu Xi;Qian Yunkai;Gao Fei;Wang Haiyang(Qinhuangdao Customs House,Qinhuangdao 066004,China)
出处
《植物检疫》
北大核心
2019年第3期13-17,共5页
Plant Quarantine
