摘要
苹果牛眼果腐病菌(Neofabraea malacorticis、N. perennans、N. kienholzii和N. vagabunda)是我国重点关注的进境水果检疫性有害生物,相似的形态和生理特征使该类真菌的鉴定非常困难。本研究在EF-1α基因区域设计了一对通用引物(NS1/NS4),对不同地理来源的苹果牛眼果腐病菌及其近缘种菌株进行序列分析,考察EF-1α基因是否具备成为苹果牛眼果腐病菌条形码的特征与条件。结果显示, EF-1α基因在苹果牛眼果腐病菌上的PCR扩增和测序成功率均为100%,具有明显的种间和种内遗传变异间距(Barcoding Gap),系统树分析显示该基因片段对4种苹果牛眼果腐病菌及其近缘种具有很好的区分能力,因此认为EF-1α基因可作为苹果牛眼果腐病菌理想的DNA条形码候选片段。
The causal pathogens of bull’s eye rot(BER)on apple(Neofabraea malicorticis,N.perennans,N.kienholzii and N.vagabunda)are important fungi of quarantine concern in China.They are difficult to distinguish from each other for their similar morphology and physiology.In this study,a pair of universal primers(NS1/NS4)was designed to amplify EF-1αregion effectively.And EF-1αwas tested with different geographical specimens of causal pathogens of bull’s eye rot and their close species to investigate its feasibility of serving as a DNA barcode.The results revealed that EF-1αhad 100%success rate of amplification and sequencing,showed obvious difference between intra-and inter-specific genetic distances(Barcoding Gap)and could effectively distinguish each species of four BER pathogens and other closely related species by phylogenetic study.Therefore,EF-1αregion could be recommended as the ideal candidate DNA barcode for the causal pathogens of bull’s eye rot on apple.
作者
林惠娇
王卫芳
Lin Huijiao;Wang Weifang(Huangpu Customs District,Guangzhou 510730,China;Guangzhou Customs District;Guangdong Key Laboratory of Import and Export Technical Measures of Animal,Plant and Food))
出处
《植物检疫》
北大核心
2019年第3期6-12,共7页
Plant Quarantine
基金
原国家质检总局科技计划项目(2014IK004)
原广东检验检疫局科技计划项目(2018GDK63)
